Ye Yuan, Zou Jingjiang, Tan Meijun, Hu Kuikui, Jiang Jindou
Department of Plastic and Cosmetic Surgery, Guangdong Women and Children Hospital, Guangzhou, China.
Front Bioeng Biotechnol. 2021 Feb 26;9:638415. doi: 10.3389/fbioe.2021.638415. eCollection 2021.
The retention of fat-derived grafts remains a challenge for regenerative medicine. Fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare a stromal vascular fraction (SVF)/extracellular matrix (ECM) gel. The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3, 14, 28, and 60 days following transplantation on the backs of nude mice. The viscosity of the fat was directly proportional to the shearing force. Although the mechanical isolation did not affect the total number of cells, it significantly decreased the number of living cells. Flow cytometry showed a greater number of mesenchymal stem cells, supra-adventitial (SA)-adipose stromal cells (ASCs), and adipose-derived stem cells but a lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat. Thus, mechanical isolation of fat can increase the pluripotency of adipocytes, which can improve graft retention in cell therapy.
脂肪源移植物的留存仍然是再生医学面临的一项挑战。将接受抽脂手术患者的脂肪抽吸物制备成标准的科尔曼脂肪移植物，或进一步利用机械剪切力进行分离，以制备基质血管成分（SVF）/细胞外基质（ECM）凝胶。在裸鼠背部移植后第3、14、28和60天，SVF/ECM凝胶的留存率显著高于科尔曼脂肪。脂肪的粘度与剪切力成正比。虽然机械分离不影响细胞总数，但显著减少了活细胞数量。流式细胞术显示，与科尔曼脂肪相比，SVF/ECM凝胶中的间充质干细胞、超外膜（SA）-脂肪基质细胞（ASC）和脂肪源性干细胞数量更多，但内皮祖细胞数量更少。因此，脂肪的机械分离可提高脂肪细胞的多能性，这有助于改善细胞治疗中的移植物留存情况。
