Sabbah A, Heulin M G, Couteleau M C, Aucouturier P, Preud'homme J L
Laboratoire d'Immuno-Allergologie, C.H.U., Angers.
Allerg Immunol (Paris). 1986 Dec;18(10):19-26.
We report on an indirect competitive immunoenzymatic assay (ELISA) for the determination of serum total IgG4 levels. This procedure allows accurate and sensitive measurements. Normal values vary according to age and sex, with mean normal levels of 22.3 +/- 14.6 mg/dl in adults. Serum IgG4 levels observed in atopic subjects are not significantly different from those in age and sex matched normal subjects. However, IgG4 levels are significantly increased in hay fever and honey bee sensitization. Measurement of serum total IgG4 levels clearly needs to be combined with the evaluation of specific anti-allergen antibodies of this subclass in investigations of the possible reaginic or blocking activity of IgG4 antibodies.
我们报告了一种用于测定血清总IgG4水平的间接竞争性免疫酶测定法（ELISA）。该方法可进行准确且灵敏的测量。正常数值因年龄和性别而异，成年人的正常平均水平为22.3±14.6mg/dl。在特应性受试者中观察到的血清IgG4水平与年龄和性别匹配的正常受试者无显著差异。然而，在花粉症和蜜蜂致敏中IgG4水平显著升高。在研究IgG4抗体可能的反应素或阻断活性时，血清总IgG4水平的测定显然需要与该亚类特异性抗变应原抗体的评估相结合。
