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采用单克隆抗体的竞争性酶免疫测定法测定正常成人血清中IgG亚类水平的分布。

Distribution of IgG subclass levels in normal adult sera as determined by a competitive enzyme immunoassay using monoclonal antibodies.

作者信息

Aucouturier P, Mounir S, Preud'homme J L

出版信息

Diagn Immunol. 1985;3(4):191-6.

PMID:4085174
Abstract

IgG subclass levels were measured by an indirect competitive immunoenzymatic assay with selected monoclonal antibodies in sera from 180 adult blood donors. Mean levels were 6.35 mg/ml (65% of the sum of the subclasses) for IgG1, 2.61 mg/ml (26.2%) for IgG2, and 0.414 mg/ml (4.3%) for IgG3. IgG4 levels were significantly different in men (mean 0.456 mg/ml, 4.8% of the sum) and women (mean 0.29 mg/ml, 3.2%), with very wide ranges. The distribution of IgG2 levels was more homogeneous than that of IgG4 but more heterogeneous than those of IgG1 and IgG3, with a number of sera that displayed low levels of IgG2.

摘要

采用间接竞争免疫酶法,使用选定的单克隆抗体,对180名成年献血者血清中的IgG亚类水平进行了测定。IgG1的平均水平为6.35毫克/毫升(占亚类总和的65%),IgG2为2.61毫克/毫升(26.2%),IgG3为0.414毫克/毫升(4.3%)。IgG4水平在男性(平均0.456毫克/毫升,占总和的4.8%)和女性(平均0.29毫克/毫升,占3.2%)中存在显著差异,范围非常广泛。IgG2水平的分布比IgG4更均匀,但比IgG1和IgG3更不均匀,有一些血清显示IgG2水平较低。

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