College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014, People's Republic of China.
Mikrochim Acta. 2024 Aug 20;191(9):549. doi: 10.1007/s00604-024-06624-6.
An intense cathodic electrochemiluminescence (ECL) is reported from a polarized glassy carbon electrode (GCE) in peroxydisulfate solution. After the polarization in 1 M NaSO at the potential of - 3.7 V for 3 s, carbon nanosheets (C-NSs) were in situ grown on the surface of the GCE. Measured in 100 mM KSO solution, the ECL intensity of the GCE/C-NSs is 112-fold that of a bare GCE. The ECL spectrum revealed that the true ECL luminophore in the GCE/C-NSs-peroxydisulfate system is O/SO which is promoted by C-NSs. When Cu was electrochemically enriched and reduced to Cu(0) on the catalytic sites of C-NSs, the ECL from GCE/C-NSs/Cu in KSO solution was decreased with increasing logarithmic concentration of Cu in the range from 10 pM to 1 μM, with a limit of detection (LOD) of 3 pM. An immunoanalysis method is proposed via a biometallization strategy using CuS nanoparticles as the tags and carcinoembryonic antigen (CEA) as the model analyte. After the immune recognition in the microplate, the CuS tags in the immunocomplex were dissolved and the resultant Cu was electrochemically enriched and reduced on the catalytic sites of C-NSs, quenching the ECL intensity of GCE/C-NSs-O/SO system. The proposed ECL immunoanalysis method was used to quantify CEA in actual serum samples with an LOD of 1.0 fg mL, possessing the advantages of simple electrode modification, high sensitivity and good reproducibility.
在过二硫酸盐溶液中，报道了一种来自极化玻碳电极（GCE）的强烈阴极电化学发光（ECL）。在 1 M NaSO 中于-3.7 V 的电位下极化 3 s 后，碳纳米片（C-NSs）原位生长在 GCE 的表面上。在 100 mM KSO 溶液中测量时，GCE/C-NSs 的 ECL 强度是裸 GCE 的 112 倍。ECL 光谱表明，在 GCE/C-NSs-过二硫酸盐体系中真正的 ECL 发光体是 O/SO，这是由 C-NSs 促进的。当 Cu 在 C-NSs 的催化位点上电化学富集并还原为 Cu(0)时，GCE/C-NSs/Cu 在 KSO 溶液中的 ECL 随着 Cu 在 10 pM 至 1 μM 范围内对数浓度的增加而降低，检测限（LOD）为 3 pM。通过使用 CuS 纳米粒子作为标记物和癌胚抗原（CEA）作为模型分析物的生物金属化策略，提出了一种免疫分析方法。在微孔板中进行免疫识别后，免疫复合物中的 CuS 标记物被溶解，所得的 Cu 在 C-NSs 的催化位点上被电化学富集和还原，猝灭 GCE/C-NSs-O/SO 体系的 ECL 强度。所提出的 ECL 免疫分析方法用于定量实际血清样品中的 CEA，检测限为 1.0 fg mL，具有电极修饰简单、灵敏度高和重现性好的优点。
