Department of Gastroenterology, The 3rd People's Hospital of Chengdu, The Second Affiliated Hospital of Chengdu, Chongqing Medical University, 82# Qinglong Street, Qingyang District, Chengdu, Sichuan, China.
National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, China.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109870. doi: 10.1016/j.msec.2019.109870. Epub 2019 Jun 6.
Endoscopic submucosal dissection (ESD) is a useful tool for the treatment of early gastric neoplasms, however post-ESD bleeding and perforation restrict its widespread application. In this study, we developed the chitosan/β-glycerophosphate/collagen (CS/GP/Col) thermo-sensitive systems to satisfy the requirements for the endoscopic treatment for ESD-induced ulcer. The results indicated that the addition of collagen to CS/GP system did not lead to remarkable changes on the physicochemical properties of the systems, which can transform from solution to hydrogels under physiological temperature within 90s, technically makes it suitable to be applied through catheter to gastric ulcer during ESD operation. Besides, hydrogels with high collagen concentration showed better biocompatibility, effectively protected L929, GES-1, HVSMC and CCD-18Co cells from acidic condition, induced more growth factors such as EGF, VEGF and FGF in those cells, and promoted coagulation. These results indicated that the CS/GP/Col thermo-sensitive hydrogel might be a promising biomaterial for the endoscopic treatment of ESD-induced ulcer, and further research can be carried out.
内镜黏膜下剥离术（ESD）是治疗早期胃癌的有效工具，然而，术后出血和穿孔限制了其广泛应用。在本研究中，我们开发了壳聚糖/β-甘油磷酸酯/胶原（CS/GP/Col）温敏体系，以满足内镜治疗 ESD 诱导溃疡的要求。结果表明，胶原的加入并未导致体系理化性质发生显著变化，该体系在生理温度下 90s 内可从溶液转变为水凝胶，这在技术上使其适用于 ESD 手术期间通过导管应用于胃溃疡。此外，高浓度胶原的水凝胶表现出更好的生物相容性，能有效保护 L929、GES-1、HVSMC 和 CCD-18Co 细胞免受酸性条件的影响，在这些细胞中诱导更多的生长因子，如 EGF、VEGF 和 FGF，并促进凝血。这些结果表明，CS/GP/Col 温敏水凝胶可能是一种有前途的内镜治疗 ESD 诱导溃疡的生物材料，可进一步研究。
