National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, Sichuan, China.
Department of Gastroenterology, The 3rd People's Hospital of Chengdu, The Second Affiliated Hospital of Chengdu, Chongqing Medical University, 82# Qinglong Street, Qingyang District, Chengdu, Sichuan, China.
Carbohydr Polym. 2019 Apr 1;209:198-206. doi: 10.1016/j.carbpol.2018.12.103. Epub 2019 Jan 2.
In order to prepare a submucosal injection agent that can gel during the endoscopic mucosal dissection (ESD) and still adhere to the wound surface after the surgery to reduce intraoperative and postoperative complications, a thermosensitive hydrogel based on chitosan/β-glycerophosphate (CS-GP) was constructed. Hydroxypropylcellulose (HPC) and collagen Ⅰ were added into the hydrogel to improve the adhesion ability, viscosity and biocompatibility. The effect of HPC on the viscosity, strength and bio-adhesion and collagen Ⅰ on the biocompatibility and promoting on the wound restoration were intensively studied. The results indicated that HPC and collagen Ⅰ will not obviously change pH of the system and the system was still physically cross-linked. HPC increased the initial gelation time but the time of the entire gelation process is shortened, especially the high-concentration HPC, and improved the mechanical strength obviously. The viscosity and the bio-adhesion of the CS-GP hydrogels were improved by the addition of HPC. Also, the mixed collagen Ⅰ offered good cytocompatibility. According to the results of cultured GES-1 cell, it can be seen that the addition of HPC and collagen Ⅰ does not inhibit cell proliferation, and shows more obvious protection on the cells under acidic conditions. All of these suggested that the application of this hydrogel were more promising in ESD surgery than which without HPC and collagen Ⅰ.
为了制备一种可在内镜黏膜下剥离（ESD）过程中凝胶化、术后仍能黏附于创面的黏膜下注射剂，以减少术中及术后并发症，构建了一种基于壳聚糖/β-甘油磷酸（CS-GP）的温敏水凝胶。向水凝胶中加入羟丙基纤维素（HPC）和胶原 Ⅰ 以提高黏附能力、黏度和生物相容性。深入研究了 HPC 对黏度、强度和生物黏附的影响，以及胶原 Ⅰ 对生物相容性和促进伤口恢复的影响。结果表明，HPC 和胶原 Ⅰ 不会明显改变体系的 pH 值，体系仍为物理交联。HPC 增加了初始胶凝时间，但整个胶凝过程的时间缩短，特别是高浓度的 HPC，并且明显提高了机械强度。HPC 的加入提高了 CS-GP 水凝胶的黏度和生物黏附性。此外，混合胶原 Ⅰ 提供了良好的细胞相容性。根据 GES-1 细胞的培养结果可以看出，添加 HPC 和胶原 Ⅰ 不会抑制细胞增殖，并且在酸性条件下对细胞表现出更明显的保护作用。所有这些都表明，与不含 HPC 和胶原 Ⅰ 的水凝胶相比，该水凝胶在 ESD 手术中的应用更有前景。
