School of Engineering, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom.
Consiglio Nazionale delle Ricerche (CNR), INO UOS 'A. Gozzini', Area della Ricerca di Pisa - S. Cataldo, via Moruzzi 1, I-56124 Pisa, Italy.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109783. doi: 10.1016/j.msec.2019.109783. Epub 2019 May 21.
In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaffolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaffolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and non-hybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaffolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro- and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the effects of these scaffolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.
在心脏组织工程（TE）中，体外模型对于研究健康和病态心脏组织至关重要，以便了解其潜在机制。在这种情况下，支架是能够真实模拟心脏自然结构的平台，并为体外模型增添生物真实性。本文报道了一种新颖而强大的技术，基于聚对二甲苯 C 和聚二甲基硅氧烷（PDMS）来制造心血管模拟支架。聚对二甲苯 C 用作掩模材料，以向 PDMS 层诱导混合和非混合微图案。混合结构呈现出条纹状的疏水性/亲水性表面，而非混合支架仅具有波纹状形貌。本文证明，PDMS 上的波浪特征可以在微纳尺度上获得，并且 PDMS 可以集成到微制造过程中而不会改变其固有物理性质。对这些支架对新生大鼠心室肌细胞（NRVMs）培养物生长影响的研究表明，只有由亲水性 PDMS 和疏水性聚对二甲苯 C 制成的混合结构才会发生细胞取向。
