Electrochemical determination of clonazepam drug based on glassy carbon electrode modified with FeO/R-SH/Pd nanocomposite.

Herein, fabrication and electrochemical evaluation of a new sensing platform, which is sensitive to clonazepam (CLZP) drug, has been illustrated. By using Fourier transform infrared spectrometry (FT-IR), electron microscopy (EM) techniques and vibrating sample magnetometer (VSM), synthesized nanocomposite, consisting of FeO, 3-aminopropyltriethoxysilane (APTES), cyanuric chloride (CC), 2-mercaptoethanol and Pd (FeO/R-SH/Pd), was characterized. Then using the common drop-casting method, the new nanocomposite was cast on the surface of bear glassy carbon electrode. The influence of effective experimental factors was investigated to optimize the voltammetric response (current) of the modified electrode for electrochemical studies and determining the CLZP. Under optimal conditions, by the designed sensor a linear relationship was found between reduction peak current and CLZP concentrations in the range of 10 nM to 1 μM CLZP, with a detection limit of 3.02 nM. This system was also employed for measuring CLZP in human serum as well as pharmaceutical samples effectively. In addition, the fabricated sensor showed good sensitivity, long-term stability, and reproducibility. Through statistical student's t-test and F-test, the insignificant systematic error was found between measured and real values and also between voltammetric method at the surface of the proposed electrode and standard HPLC method.