Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2018 Sep;116:966-976. doi: 10.1016/j.ijbiomac.2018.05.102. Epub 2018 May 18.
Interconnected microporous biodegradable and biocompatible chitin/silk fibroin/TiO nanocomposite wound dressing with high antibacterial, blood clotting and mechanical strength properties were synthesized using freeze-drying method. The prepared nanocomposite dressings were characterized using SEM, FTIR, and XRD analysis. The prepared nanocomposite dressings showed high porosity above 90% with well-defined interconnected porous construction. Swelling and water uptake of the dressing were 93%, which is great for wound dressing applications. Haemostatic potential of the prepared dressings was studied and the results proved the higher blood clotting ability of the nanocomposites compared to pure components and commercially available products. Besides, cell viability, attachment and proliferation by MTT assay and DAPI staining on HFFF2 cell as a Human Caucasian Foetal Foreskin Fibroblast proved the cytocompatibility nature of the nanocomposite scaffolds with well improved proliferation and cell attachment. To determine the antimicrobial efficiencies, both disc diffusion method and colony counts were performed and results imply that nanocomposite scaffolds have high antimicrobial activity and could successfully inhibit the growth of E. coli, S. aureus, and C. albicans. Moreover, based on these results, the prepared chitin/silk fibroin/TiO nanocomposite dressing could serve as a kind of promising wound dressing with great antibacterial and antifungal properties.
采用冷冻干燥法合成了具有高抗菌、止血和机械强度性能的互穿微孔可生物降解和生物相容的甲壳素/丝素纤维/TiO 纳米复合材料伤口敷料。使用 SEM、FTIR 和 XRD 分析对制备的纳米复合材料敷料进行了表征。所制备的纳米复合材料敷料的孔隙率高于 90%，具有良好的定义的互穿多孔结构。敷料的溶胀率和吸水率为 93%，非常适合伤口敷料应用。研究了制备敷料的止血潜力，结果表明纳米复合材料比纯成分和市售产品具有更高的凝血能力。此外，通过 MTT 分析和 DAPI 染色在 HFFF2 细胞（人白种胎儿包皮成纤维细胞）上进行的细胞活力、附着和增殖证明了纳米复合材料支架的细胞相容性，其增殖和细胞附着得到了很好的改善。为了确定抗菌效率，进行了圆盘扩散法和菌落计数，结果表明纳米复合材料支架具有很高的抗菌活性，可以成功抑制大肠杆菌、金黄色葡萄球菌和白色念珠菌的生长。此外，基于这些结果，所制备的甲壳素/丝素纤维/TiO 纳米复合材料敷料可以作为一种具有良好抗菌和抗真菌性能的有前途的伤口敷料。
