Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay (IIT Bombay), Mumbai, India.
Center for Research in Nanotechnology & Science (CRNTS), IIT Bombay, Mumbai, India.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109774. doi: 10.1016/j.msec.2019.109774. Epub 2019 May 18.
Currently, an enormous amount of cancer research based on two-dimensional nano-graphene oxide (GO), as well as zero-dimensional graphene quantum dots (GQDs), is being carried out in the fields of therapeutics and diagnostics. However, the exploration of their hybrid "functional" nanomaterials in the theranostic system is still rare. In the current study, a stable complex of GO and GQDs was formed by an electrostatic layer-by-layer assembly via a polyethylene imine bridge (GO-PEI-GQDs). Furthermore, we compared separate mono-equivalents of the GO-PEI-GQDs complex - GO and GQDs, in terms of cell imaging (diagnostics), photothermal, and oxidative stress response in breast cancer cells (MDA-MB-231). GO-PEI-GQDs showed an excellent photothermal response (44-49 °C) upon 808 nm laser (0.5 W cm) exposure for 5 min at a concentration up to 50 μg/mL. We report new synergistic properties of GO-PEI-GQDs such as stable fluorescence imaging and enhanced photothermal and cytotoxic activities on cancer cells. Composite materials made up of GO and GQDs combining diverse properties help to study 2D-0D heterosystems and improve specific therapeutic systems in theranostics.
目前，在治疗学和诊断学领域，基于二维纳米氧化石墨烯（GO）和零维石墨烯量子点（GQDs）的大量癌症研究正在进行。然而，它们在治疗系统中混合“功能”纳米材料的探索仍然很少。在当前的研究中，通过聚亚乙基亚胺桥（GO-PEI-GQDs）的静电层层组装形成了 GO 和 GQDs 的稳定复合物。此外，我们比较了 GO-PEI-GQDs 复合物的单独单当量 - GO 和 GQDs，在乳腺癌细胞（MDA-MB-231）中的细胞成像（诊断）、光热和氧化应激反应方面。GO-PEI-GQDs 在 808nm 激光（0.5W/cm）照射下，浓度高达 50μg/mL 时，在 5min 内表现出出色的光热响应（44-49°C）。我们报告了 GO-PEI-GQDs 的新协同特性，例如稳定的荧光成像以及增强的光热和细胞毒性活性对癌细胞的影响。由 GO 和 GQDs 组成的复合材料结合了多种特性，有助于研究 2D-0D 异质系统并改善治疗学中的特定治疗系统。
