Textile Industerial Research Division, National Research Centre (Scopus Affiliation ID 60014618), El-Bohouth St., Dokki, P.O. 12622, Giza, Egypt.
Chemistry of Natural and Microbial Products Dept., National Research Centre, Dokki, Giza, Egypt; Biology Dept., Faculty of Education, Hafr Al Batin University, Saudi Arabia.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):2121-2130. doi: 10.1016/j.ijbiomac.2018.07.062. Epub 2018 Jul 20.
In the realm of green synthesis of metals nanoparticles for medical textile application, silver nanoparticles (AgNPs) were biosynthesized in situ cotton fabrics for the first time by using fungi for rendering cotton fabrics antimicrobial activity with abroad range towards different pathogenic organisms. Herein, five different isolated fungi from medicinal plants were identified and optimized their growth media prior examined their ability to reduce Ag ions to AgNPs in-situ cotton fabrics along with ex-situ method. Synthesis of AgNPs were characterized by making use of instruments e.g. UV-vis spectroscopy, Transmission Electron Microscopy (TEM), Selected Area Electron Diffraction (SAED), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared (FTIR). Whereas antimicrobial activities of the resultant cotton fabrics were investigated against Gram positive (S. aureus ATCC29213), Gram negative (E. coli ATCC 25922), Yeast (C. albicans ATCC10321) and, fungi (A. niger NRC 53). Results revealed the successful biosynthesis of AgNPs using different fungus strains whether in-situ cotton fabrics or ex-situ manner. The size of the resultant AgNPs by ex-situ method were varied (5-20 nm). The antimicrobial activity of the in-situ treated cotton samples exhibited different behaviors towards both pathogenic bacteria and fungi. This manner opens up a new way to discover the ability of nanobiotechnology to provide world with substitutional aids mimic to synthetic materials.
在医学纺织品应用的金属纳米粒子绿色合成领域，首次通过真菌原位合成将银纳米粒子 (AgNPs) 生物合成到棉织物中，使棉织物具有广谱的抗菌活性，针对不同的病原体。在此，从药用植物中分离出的五种不同真菌被鉴定出来，并对其生长培养基进行了优化，然后检查了它们在原位和异位方法下将银离子还原为 AgNPs 的能力。AgNPs 的合成通过使用仪器进行表征，例如 UV-vis 光谱、透射电子显微镜 (TEM)、选区电子衍射 (SAED)、扫描电子显微镜 (SEM) 和傅里叶变换红外 (FTIR)。而所得棉织物的抗菌活性则针对革兰氏阳性菌 (S. aureus ATCC29213)、革兰氏阴性菌 (E. coli ATCC 25922)、酵母 (C. albicans ATCC10321) 和真菌 (A. niger NRC 53) 进行了研究。结果表明，使用不同的真菌菌株成功地原位合成了 AgNPs 或异位合成了 AgNPs。通过异位法获得的 AgNPs 的大小不同（5-20nm）。原位处理的棉样的抗菌活性对病原菌和真菌表现出不同的行为。这种方式开辟了一条新的途径，可以发现纳米生物技术为世界提供替代合成材料的模拟物的能力。
