Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China.
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Int J Mol Sci. 2019 Jul 25;20(15):3632. doi: 10.3390/ijms20153632.
2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89-10.44 mg/L. The larvae treated with 2,4-DNT showed toxic symptoms including smaller body, less skin pigment production, yolk malabsorption, and disordered liver development. Further studies on the expression of genes related to lipid transport and metabolism, and respiration indicated that they were significantly affected by 2,4-DNT. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression.
2,4-二硝基甲苯（2,4-DNT）是一种常见的环境污染物，国际癌症研究机构将其归类为 2B 类人类致癌化合物。本研究以胚胎-幼虫阶段的斑马鱼为研究对象，探讨了 2,4-DNT 暴露对其的毒性作用，主要表现在器官形态发生和与脂质代谢及氧气运输相关的选定靶基因的表达模式上。结果表明，2,4-DNT 的 120 小时受精后半数致死浓度（LC）为 9.59 毫克/升，95%置信区间为 8.89-10.44 毫克/升。经 2,4-DNT 处理的幼虫表现出毒性症状，包括身体较小、皮肤色素生成减少、卵黄吸收不良和肝脏发育紊乱。进一步研究与脂质转运和代谢以及呼吸相关的基因表达表明，它们受到 2,4-DNT 的显著影响。综上所述，2,4-DNT 暴露扰乱了早期生活斑马鱼的肝脏发育和卵黄吸收，干扰了脂质代谢/氧气运输基因的表达。
