穿孔 SERS 活性阵列的制备与特性研究及其在颗粒捕获和灵敏分子分析中的应用。

Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Konkoly-Thege Miklós út 29-33., HAS, 1121 Budapest, Hungary.

Institute of Technical Physics and Materials Science, Centre for Energy Research, Konkoly-Thege Miklós út 29-33., HAS, 1121 Budapest, Hungary.

Biosensors (Basel). 2019 Jul 25;9(3):93. doi: 10.3390/bios9030093.

A gold-coated array of flow-through inverse pyramids applicable as substrate for entrapment and immobilization of micro-objects and for surface enhanced Raman spectroscopic measurements was fabricated using bulk micromachining techniques from silicon. Surface morphology, optical reflectance, immobilization properties, and surface enhanced Raman amplification of the array were modelled and characterized. It was found that the special perforated periodic 3D structure can be used for parallel particle and cell trapping and highly sensitive molecular analysis of the immobilized objects.

采用体硅微加工技术制作了一种金涂覆的流通式倒金字塔阵列，可用作微物体的捕获和固定以及表面增强拉曼光谱测量的基底。对该阵列的表面形貌、光学反射率、固定性能和表面增强拉曼放大进行了建模和表征。研究发现，特殊的穿孔周期性 3D 结构可用于并行的粒子和细胞捕获，以及对固定物体进行高灵敏度的分子分析。