College of Food Science and Nutritional Engineering , China Agricultural University , Beijing , 100083 , China.
Department of Food Science , Purdue University , West Lafayette , Indiana 47906 , United States.
J Agric Food Chem. 2019 Apr 24;67(16):4603-4610. doi: 10.1021/acs.jafc.8b07317. Epub 2019 Apr 15.
In this paper, a biodegradable gold coated zein film surface enhanced Raman spectroscopy (SERS) platform, with gold nanoparticles (AuNPs) deposited on the surface to further enhance the Raman signal, was used to detect pyocyanin (PYO), the toxin secreted by Pseudomonas aeruginosa. An inverted pyramid structure imprinted on a zein film and gold coated during the transfer process was further improved with the deposition and fixing of gold nanoparticles, which resulted in enhancement of the SERS signal by approximately a decade. This new platform served as a lab-on-a-chip sensor to enable the sensitive and rapid detection of PYO in drinking water. The size, distribution, and morphology of the zein film nanostructures including the presence and distribution of gold nanoparticles were characterized by scanning electron microscopy (SEM). The new zein-based platform has the advantage of being largely biodegradable compared with commercial silicon- or glass-based platforms. The limit of detection for PYO using the newly developed zein-based SERS sensor platform was calculated as 25 μM, considerably lower than the concentration of PYO in the blood of people with cystic fibrosis which has been reported to be 70 μM.
在本文中，我们构建了一种可生物降解的金包裹玉米醇溶蛋白薄膜表面增强拉曼光谱（SERS）平台，在该平台上沉积了金纳米粒子（AuNPs）以进一步增强拉曼信号，用于检测铜绿假单胞菌（Pseudomonas aeruginosa）分泌的毒素绿脓菌素（PYO）。在转移过程中，在玉米醇溶蛋白薄膜上压印的倒金字塔结构进一步通过沉积和固定金纳米粒子得到了改善，这导致 SERS 信号增强了约十倍。该新型平台作为一种芯片实验室传感器，可实现饮用水中 PYO 的灵敏快速检测。扫描电子显微镜（SEM）用于表征玉米醇溶蛋白薄膜纳米结构的大小、分布和形态，包括金纳米粒子的存在和分布。与商用硅基或玻璃基平台相比，新型玉米醇溶蛋白基平台具有很大的生物降解优势。使用新开发的基于玉米醇溶蛋白的 SERS 传感器平台检测 PYO 的检出限计算为 25 μM，明显低于囊性纤维化患者血液中报道的 PYO 浓度（70 μM）。
