Institute of Plant Biology and Biotechnology, University of Muenster, Schlossplatz 8, 48143 Muenster, Germany.
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schlossplatz 8, 48143 Muenster, Germany.
Molecules. 2019 Jul 25;24(15):2703. doi: 10.3390/molecules24152703.
In addition to natural rubber (NR), several triterpenes are synthesized in laticifers of the Russian dandelion (). Detailed analysis of NR and resin contents revealed different concentrations of various pentacyclic triterpenes such as α-, β-amyrin and taraxasterol, which strongly affect the mechanical properties of the resulting rubber material. Therefore, the reduction of triterpene content would certainly improve the industrial applications of dandelion NR. We developed plants with reduced triterpene contents by tissue-specific downregulation of major laticifer-specific oxidosqualene cyclases (OSCs) by RNA interference, resulting in an almost 67% reduction in the triterpene content of NR. Plants of the T1 generation showed no obvious phenotypic changes and the rubber yield also remained unaffected. Hence, this study will provide a solid basis for subsequent modern breeding programs to develop Russian dandelion plants with low and stable triterpene levels.
除了天然橡胶 (NR)，几种三萜类化合物也在俄罗斯蒲公英的乳汁管中合成（）。对 NR 和树脂含量的详细分析揭示了各种五环三萜的不同浓度，如 α-、β-香树脂醇和蒲公英甾醇，它们强烈影响橡胶材料的机械性能。因此，降低三萜含量肯定会改善蒲公英 NR 的工业应用。我们通过 RNA 干扰特异性下调主要乳管特异性角鲨烯环氧化酶 (OSC)，开发出三萜含量降低的植物，导致 NR 中的三萜含量降低近 67%。T1 代植物没有明显的表型变化，橡胶产量也没有受到影响。因此，本研究将为随后的现代育种计划提供坚实的基础，以开发具有低而稳定三萜水平的俄罗斯蒲公英植物。
