Lu Yixiao, Sengvilaipaseuth Onanong, Chanthongthip Anisone, Phonemixay Ooyanong, Vongsouvath Manivanh, Phouminh Phonelavanh, Blacksell Stuart D, Newton Paul N, Dubot-Pérès Audrey
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit (LOMWRU), Microbiology Laboratory, Mahosot Hospital, Vientiane 01000, Laos.
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Trop Med Infect Dis. 2019 Jul 25;4(3):111. doi: 10.3390/tropicalmed4030111.
The endemicity of Dengue virus (DENV) infection remains a major public health problem in Lao PDR. In this study, we compared two commercial anti-dengue IgM ELISA kits, Panbio Dengue IgM Capture ELISA (Panbio Kit, Alere, Waltham, MA, USA) and DEN Detect MAC-ELISA (InBios kit, InBios International, Inc., Seattle, WA, USA), in the context of diagnosis of patients admitted to hospital with clinical dengue presentation. Two panels of paired blood samples were tested. Panel A was composed of 54 dengue confirmed patients (by DENV real-time RT-PCR) and 11 non-dengue dengue patients (other infections confirmed by corresponding PCR results). Panel B included 74 patients randomly selected from consecutive patients admitted to Mahosot Hospital in 2008 with suspicion of dengue fever according to WHO criteria. Results from panel A showed significantly better sensitivity for Panbio kit (64.8%; 95%CI: 50.6-77.3%) than for InBios kit (18.5%; 95%CI: 9.3-31.4%) when testing admission sera. Sensitivity was increased for both kits when combining results from admission and convalescent sera. Concordant results were obtained from panel B with fair agreement (κ = 0.29) between both kits when testing single admission samples, and moderate agreement (κ = 0.5) when combining results from admission and convalescent sera.
登革热病毒（DENV）感染的地方性流行仍是老挝人民民主共和国的一个主要公共卫生问题。在本研究中，我们比较了两种商用抗登革热IgM ELISA试剂盒，即泛博生物登革热IgM捕获ELISA试剂盒（泛博试剂盒，美国马萨诸塞州沃尔瑟姆市阿莱公司）和DEN Detect MAC-ELISA试剂盒（英生物试剂盒，美国华盛顿州西雅图市英生物国际公司），用于诊断因临床登革热症状入院的患者。对两组配对血样进行了检测。A组由54例登革热确诊患者（通过DENV实时RT-PCR确诊）和11例非登革热患者（通过相应PCR结果确诊为其他感染）组成。B组包括2008年根据世界卫生组织标准从万象Mahosot医院连续收治的疑似登革热患者中随机选取的74例患者。A组结果显示，检测入院血清时，泛博试剂盒的灵敏度（64.8%；95%CI：50.6-77.3%）显著高于英生物试剂盒（18.5%；95%CI：9.3-31.4%）。将入院血清和恢复期血清的结果相结合时，两种试剂盒的灵敏度均有所提高。B组检测单一入院样本时，两种试剂盒的结果一致，一致性一般（κ = 0.29）；将入院血清和恢复期血清的结果相结合时，一致性中等（κ = 0.5）。
