Taha Ayman E, AbdAllah Osama A, Attia Khalil M, El-Karim Ragaa E Abd, El-Hack Mohamed E Abd, El-Edel Mohamed A, Saadeldin Islam M, Hussein Elsayed O S, Swelum Ayman A
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Behira, Rasheed, Edfina 22758, Egypt.
Animal Production Research Institute, Ministry of Agriculture, Dokki, Cairo 12618, Egypt.
Animals (Basel). 2019 Jul 25;9(8):486. doi: 10.3390/ani9080486.
The hypothesis of the present work was that the effects of in ovo injection may differ in different chicken strains. The influence of in ovo royal jelly (RJ) injection on hatching, growth and blood parameters in two chicken strains (Dokki-4 and El-Salam as example for different strains) was evaluated. A total of 1080 eggs were used. On the seventh day of incubation, the eggs were randomly allocated into six experimental groups in a 2 × 3 arrangement that included the two chicken strains and three concentrations of RJ (0, 0.25 and 0.5 mL RJ/egg). Injection with 0.5 mL RJ/egg improved hatchability compared to the other treatments. The El-Salam strain exhibited significantly higher body weight and body weight gain than the Dokki-4 strain. Injection with 0.5 mL RJ/egg significantly ( < 0.05) improved chicken body weight and daily weight gain compared to the control treatment. RJ injection decreased blood lipid profile parameters and the numbers of monocytes and eosinophils and increased total protein, globulin, haemoglobin (Hb) and lymphocyte levels compared to the control treatment. The Dokki-4 strain showed significantly higher antibody titres against avian influenza virus (AIV) ( < 0.05) and sheep red blood cells (SRBCs) ( < 0.0001) than the El-Salam strain and RJ injection enhanced antibody titres against AIV, Newcastle disease virus (NDV) and SRBCs. Therefore, the Dokki-4 strain was superior to the El-Salam strain for the tested parameters and injection with 0.5 mL RJ/egg produced the best hatching parameters, growth performance and health-related traits. RJ in ovo injection was much more effective in the Dokki-4 strain than in the El-Salam strain, which supported the hypothesis of the study that varying the chicken strain could alter the response to the in ovo injection with RJ.
本研究的假设是，胚内注射的效果在不同鸡种中可能存在差异。评估了胚内注射蜂王浆（RJ）对两种鸡种（以Dokki-4和El-Salam作为不同鸡种的示例）孵化、生长和血液参数的影响。总共使用了1080枚鸡蛋。在孵化的第7天，将鸡蛋按照2×3的排列方式随机分为六个实验组，其中包括两种鸡种和三种浓度的RJ（0、0.25和0.5 mL RJ/枚鸡蛋）。与其他处理相比，注射0.5 mL RJ/枚鸡蛋提高了孵化率。El-Salam鸡种的体重和体重增加显著高于Dokki-4鸡种。与对照处理相比，注射0.5 mL RJ/枚鸡蛋显著（P<0.05）提高了鸡的体重和日增重。与对照处理相比，RJ注射降低了血脂谱参数以及单核细胞和嗜酸性粒细胞数量，并提高了总蛋白、球蛋白、血红蛋白（Hb）和淋巴细胞水平。Dokki-4鸡种针对禽流感病毒（AIV）（P<0.05）和绵羊红细胞（SRBCs）（P<0.0001）的抗体效价显著高于El-Salam鸡种，并且RJ注射增强了针对AIV、新城疫病毒（NDV）和SRBCs的抗体效价。因此，在所测试的参数方面，Dokki-4鸡种优于El-Salam鸡种，并且注射0.5 mL RJ/枚鸡蛋产生了最佳的孵化参数、生长性能和与健康相关的性状。胚内注射RJ在Dokki-4鸡种中比在El-Salam鸡种中更有效，这支持了该研究的假设，即改变鸡种可能会改变对胚内注射RJ的反应。
