Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), 333 Techno Jungang Daero, Hyeonpung-Eup, Dalseong-Gun, Daegu 42988, Republic of Korea.
Graduate School of Analytical Science and Technology (GRAST), Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Biointerphases. 2019 Jul 26;14(4):041001. doi: 10.1116/1.5099563.
When a tissue slice pretreated with gold nanoparticles is irradiated with a focused 532-nm continuous wave laser, desorption is observed to be uniform and stable, and its shape is sharp and steep. However, since the desorbed molecules are still electrically neutral particles in atmospheric pressure (AP), additional procedure of ionizing them is necessary for AP mass spectrometry (AP-MS) analysis. Therefore, the authors have combined an electrospray device with a simple chamber connected to the airflow-assisted particle transport equipment mounted at the mass spectrometer inlet. Subsequent ionization processes using an electrospray device enable the detection of several types of diacylglycerol molecules above 500 Da, which cannot be detected with the use of AP plasma jets. The authors also developed a remote AP-MS using a long and flexible sampling probe and a fiber laser with a slight modification of the proposed AP desorption and ionization method.
当用聚焦的 532nm 连续波激光辐照用金纳米粒子预处理的组织切片时，观察到解吸是均匀和稳定的，并且其形状尖锐陡峭。然而，由于在大气压 (AP) 下解吸的分子仍然是电中性的粒子，因此对于 AP 质谱分析 (AP-MS) 分析，需要对它们进行额外的电离过程。因此，作者将电喷雾装置与一个简单的腔室相结合，该腔室连接到安装在质谱仪入口处的气流辅助颗粒输送设备。使用电喷雾装置进行后续的电离过程可以检测到几种超过 500Da 的二酰基甘油分子，而使用 AP 等离子体射流则无法检测到这些分子。作者还使用长而灵活的采样探针和光纤激光器开发了一种远程 AP-MS，对所提出的 AP 解吸和电离方法进行了轻微修改。
