Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6DX, U.K.
Anal Chem. 2024 Oct 8;96(40):15865-15870. doi: 10.1021/acs.analchem.4c04458. Epub 2024 Sep 27.
A liquid atmospheric pressure-matrix-assisted laser desorption/ionization (LAP-MALDI) method has been developed and applied to a commercial AP-MALDI source on a hybrid orbitrap mass analyzer. It is shown that electrospray ionization (ESI)-like mass spectra of a range of peptides and proteins can be acquired by LAP-MALDI mass spectrometry (MS) as previously demonstrated on a homemade LAP-MALDI-Q-TOF setup but without the need of any modification to the commercially available MS equipment used. Multiply charged peptide ions were recorded with a resolution of around 100,000 and a mass accuracy of less than 5 ppm. The higher resolution and mass accuracy of the orbitrap analyzer compared with previously employed Q-TOF instrumentation provided high confidence in bacterial proteoform and species identification by top-down protein analysis.
已经开发出一种液相常压基质辅助激光解吸/电离（LAP-MALDI）方法，并将其应用于混合轨道阱质谱仪上的商用 AP-MALDI 源。结果表明，如先前在自制的 LAP-MALDI-Q-TOF 装置上所证明的那样，通过 LAP-MALDI 质谱（MS）可以获得一系列肽和蛋白质的类似于电喷雾电离（ESI）的质谱，而无需对所使用的市售 MS 设备进行任何修改。多电荷肽离子的分辨率约为 100,000，质量精度小于 5 ppm。与先前使用的 Q-TOF 仪器相比，轨道阱分析仪的更高分辨率和质量精度为通过自上而下的蛋白质分析对细菌蛋白形式和物种进行鉴定提供了高度信心。
