Mehrzad Jamshid, Dayyani Mahdieh, Khorasani Mohammadreza Erfanian
Department of Biochemistry, Neyshabur Medical Sciences Branch, Islamic Azad University, Neyshabur, Iran. Email:
Department of Radiation Oncology, Reza Radiotherapy Oncology Center, Mashhad, Iran.
Asian Pac J Cancer Prev. 2019 Jul 1;20(7):2153-2158. doi: 10.31557/APJCP.2019.20.7.2153.
Background: Colorectal cancer (CRC) is highly prevalent cancer, which should be genetically studied among different peoples of the world. Objective: The aim of this study was to evaluate the effect of XRCC3T241M, XRCC3 A17893G and, for the first time, XRCC7 I3434T polymorphisms on CRC risk in Khorasan Razavi Province, Iran. Materials and Methods: In this case-control study, 180 patients with CRC and 160 sex- and age-matched healthy controls were studied. Genotypes were determined by RFLP-PCR and ARMS-PCR. Results: The incidence of CRC was observed to be significantly more in a heterozygous XRCC3 C/T genotype than in the CC genotype (OR 2.210, 95% CI 1.073-4.548, p=0.030). In the case of the XRCC7 I3434T polymorphism, CRC risk was significantly (4.3 fold) higher in I/T+T/T variant subjects compared to the I/I genotype (OR 4.394, 95% CI 2.721-7.096, p=0.000). Moreover, the XRCC3 A17893G polymorphism did not correlate with CRC. In addition, there was no significant difference between the distribution of genotypes of the three studied polymorphisms with demographic and clinicopathological features in the CRC patients. Conclusion: Polymorphisms of XRRC3 and XRCC7 genes are involved in CRC and should be considered as a risk factor.
结直肠癌（CRC）是一种高度常见的癌症，应对世界不同人群进行基因研究。目的：本研究旨在评估XRCC3 T241M、XRCC3 A17893G以及首次研究的XRCC7 I3434T多态性对伊朗呼罗珊拉扎维省结直肠癌风险的影响。材料与方法：在这项病例对照研究中，对180例结直肠癌患者和160例年龄及性别匹配的健康对照进行了研究。通过限制性片段长度多态性聚合酶链反应（RFLP-PCR）和扩增阻滞突变系统聚合酶链反应（ARMS-PCR）确定基因型。结果：观察到杂合子XRCC3 C/T基因型的结直肠癌发病率显著高于CC基因型（比值比2.210，95%置信区间1.073 - 4.548，p = 0.030）。就XRCC7 I3434T多态性而言，与I/I基因型相比，I/T + T/T变异受试者的结直肠癌风险显著更高（高4.3倍）（比值比4.394，95%置信区间2.721 - 7.096，p = 0.000）。此外，XRCC3 A17893G多态性与结直肠癌无关。另外，在结直肠癌患者中，所研究的三种多态性的基因型分布与人口统计学和临床病理特征之间无显著差异。结论：XRRC3和XRCC7基因的多态性与结直肠癌有关，应被视为一个风险因素。
