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无烟烟草依赖对男性志愿者红细胞和血小板膜的影响：一项观察研究

Possible Association of Smokeless Tobacco Dependent Impairment in the Erythrocytes and Platelets Membranes of Human Male Volunteers: An Observation.

作者信息

Shaik Fareeda Begum, Nagajothi G, Swarnalatha K, Kumar C Vinod, Dhania K Narender, Kumar C Suresh, Maddu Narendra

机构信息

Department of Biochemistry, Sri Krishnadevaraya University, Ananthapuramu, Andhra Pradesh, India. Email:

Department of Corporate Secretary ship, Queen Mary's College (Autonomous), Chennai, Tamil Nadu, India.

出版信息

Asian Pac J Cancer Prev. 2019 Jul 1;20(7):2167-2176. doi: 10.31557/APJCP.2019.20.7.2167.

DOI:10.31557/APJCP.2019.20.7.2167
PMID:31350981
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6745197/
Abstract

Background: Smokeless tobacco (SLT) acts as a modifier of erythrocyte and platelet membranes by disrupting antioxidant system with the concomitant increase in free radical production and induction of apoptosis. Methods: The SLT users was that individuals used gutkha and khaini products (Khaleja/mahak chaini brand respectively) habitually, at least >20 times per week consists of 50-60 g during the last 2-4 years. Results: The gutkha and khaini users found to be significantly increased levels of iNOS (Inducible nitric oxide synthase) enzyme in plasma, erythrocytes, and platelet membranes when compared to normal controls. The gutkha and khaini users exhibited that the significant increase in the levels of gene expression of apoptotic proteins (Bcl2-B cell lymphoma gene 2, Bax, caspases 8, caspase 10, and caspase 12), IL-6 (Interleukin-6), and decreased levels of TNF-α (Tumor necrosis factor-alpha) and decreased expression of caspase 12 of khaini users were observed from blood samples. The significant increase in the concentrations of peroxynitrites (ONOO-), nitric oxide (NO) (Nitrates and nitrites), malondialdehyde (MDA), cholesterol, and phospholipids were reported in the smokeless tobacco users of erythrocytes and platelets. The experimental subjects showed that the increased osmotic fragility and decreased membrane fluidity of erythrocytes and platelets in comparison with non-tobacco users. The normal subjects had been exposed that the proper functioning of antioxidant enzymes and decreased enzyme activities of antioxidants were reported by SLT users. Conclusion: The smokeless tobacco products are exerted chronic damage to membranes of erythrocytes and platelets and elevation of apoptosis in the prolonged periods of human male volunteers.

摘要

背景

无烟烟草（SLT）通过破坏抗氧化系统，伴随自由基产生增加和诱导细胞凋亡，对红细胞和血小板膜起修饰作用。方法：SLT使用者为习惯性使用古特卡和哈伊尼产品（分别为Khaleja/马哈克查尼品牌）的个体，在过去2至4年中每周至少使用>20次，每次用量为50 - 60克。结果：与正常对照组相比，发现古特卡和哈伊尼使用者血浆、红细胞和血小板膜中诱导型一氧化氮合酶（iNOS）水平显著升高。从古特卡和哈伊尼使用者的血液样本中观察到，凋亡蛋白（Bcl2 - B细胞淋巴瘤基因2、Bax、半胱天冬酶8、半胱天冬酶10和半胱天冬酶12）、白细胞介素 - 6（IL - 6）的基因表达水平显著升高，哈伊尼使用者的肿瘤坏死因子 - α（TNF - α）水平降低且半胱天冬酶12表达减少。无烟烟草使用者的红细胞和血小板中过氧亚硝酸盐（ONOO - ）、一氧化氮（NO）（硝酸盐和亚硝酸盐）、丙二醛（MDA）、胆固醇和磷脂的浓度显著升高。与非烟草使用者相比，实验对象的红细胞和血小板渗透脆性增加，膜流动性降低。正常受试者暴露于抗氧化酶功能正常的环境中，而SLT使用者报告抗氧化酶活性降低。结论：无烟烟草产品对人类男性志愿者的红细胞和血小板膜造成慢性损伤，并在较长时间内导致细胞凋亡增加。

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