PITX3-mCherry基因敲入报告基因人胚胎干细胞系（WAe009-A-23）的建立。
Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23).
作者信息
Park Sanghyun, Yoo Jeong-Eun, Lee Dongjin R, Jang Jiho, Cho Myung Soo, Kim Dae-Sung, Kim Dong-Wook
机构信息
Department of Physiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea; Brain Korea 21 PLUS Program for Medical Science, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
Department of Physiology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea; Severance Biomedical Research Institute, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, South Korea.
出版信息
Stem Cell Res. 2019 Aug;39:101499. doi: 10.1016/j.scr.2019.101499. Epub 2019 Jul 18.
Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.
垂体同源框3（Pitx3）是一种关键转录因子，在中脑多巴胺能（mDA）神经元的发育和维持中发挥重要作用。在此，我们利用CRISPR/Cas9系统建立了一个PITX3- mCherry敲入报告基因人类胚胎干细胞（hESC）系。PITX3- mCherry hESCs维持多能性标志物表达，并表现出产生所有三个胚层和正常核型的能力。分化为mDA神经元后，大多数PITX3免疫反应性与mCherry的红色荧光重叠。该报告细胞系可用于研究mDA神经元的发育或富集用于移植的mDA群体。
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