Department of Cardiovascular Surgery and Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
Department of Cardiovascular Surgery, The Affiliated Hospital, Southwest Medical University, Metabolic Vascular Diseases Key Laboratory of Sichuan Province, Key Laboratory of Cardiovascular Remodeling and Dysfunction, Luzhou 646000, PR China; Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases), Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, Sichuan, PR China.
Stem Cell Res. 2024 Apr;76:103376. doi: 10.1016/j.scr.2024.103376. Epub 2024 Mar 4.
The ISL LIM homeobox 1 (ISL1) gene belongs to the LIM/homeodomain transcription factor family and plays a pivotal role in conveying multipotent and proliferative properties of cardiac precursor cells. Mutations in ISL1 are linked to congenital heart disease. To further explore ISL1's role in the human heart, we have created a homozygous ISL1 knockout (ISL1-KO) human embryonic stem cell line using the CRISPR/Cas9 system. Notably, this ISL1-KO cell line retains normal morphology, pluripotency, and karyotype. This resource serves as a valuable tool for investigating ISL1's function in cardiomyocyte differentiation.
ISL LIM 同源盒 1（ISL1）基因属于 LIM/同源结构域转录因子家族，在传递心脏前体细胞的多能性和增殖特性方面发挥着关键作用。ISL1 基因突变与先天性心脏病有关。为了进一步探索 ISL1 在人类心脏中的作用，我们使用 CRISPR/Cas9 系统创建了一个纯合的 ISL1 敲除（ISL1-KO）人胚胎干细胞系。值得注意的是，这种 ISL1-KO 细胞系保持正常的形态、多能性和核型。该资源是研究 ISL1 在心肌细胞分化中的功能的有力工具。
