Ojo Oluwafemi Adeleke, Afon Adeola Agnes, Ojo Adebola Busola, Ajiboye Basiru Olaitan, Okesola Mary Abiola, Aruleba Raphael Taiwo, Adekiya Tayo Alex, Oyinloye Babatunji Emmanuel
Phytomedicine, and Biomedical Toxicology Unit, Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
J Food Biochem. 2019 Mar;43(3):e12772. doi: 10.1111/jfbc.12772. Epub 2019 Jan 20.
Due to the exceptional wide range in biochemical activities of natural plant products, Spondias mombim L. are attaining a new height because they present great prospects for drug advancement. This research was designed to analyze the pharmaceutical properties of S. mombim L. ethyl acetate fraction (SMEAF) on key enzymes relevant to erectile and cognitive dysfunction. SMEAF inhibitory activities of the specified enzymes were determined spectrophotometrically. Chemical profile of SMEAF were assessed by HPLC/MS analysis. Thereafter, molecular docking of the studied enzymes with chlorogenic acid, lutein, and zeaxanthin were carried out using PATCHDOCK. SMEAF had remarkable enzyme inhibitory effects against phosphodiesterase-5 (PDE-5), arginase, angiotensin I-converting enzyme (ACE), cholinesterase, monoamine oxidase A (MAO), ecto-5' nucleotidase (E-NTDase), tyrosinase, and stimulated sodium-potassium ATPase (Na+/K+-ATPase) activities. HPLC/MS analysis revealed that phenolics and carotenoids were major components in these fraction notably, chlorogenic acid, lutein, and zeaxanthin. Our results suggested that SMEAF could be explored as phytopharmaceuticals. PRACTICAL APPLICATIONS: Spondias mombim L. are cooked as green vegetable with enormous medicinal value probably due to its polyphenols with potent antioxidant activity. Furthermore, the leaves could also be useful for therapeutic purposes against erectile dysfunction and central nervous system disorders.
由于天然植物产品具有异常广泛的生化活性，腰果树正迎来新的发展高度，因为它们在药物研发方面具有巨大潜力。本研究旨在分析腰果树乙酸乙酯馏分（SMEAF）对与勃起功能障碍和认知功能障碍相关的关键酶的药理特性。通过分光光度法测定SMEAF对特定酶的抑制活性。通过HPLC/MS分析评估SMEAF的化学特征。此后，使用PATCHDOCK对所研究的酶与绿原酸、叶黄素和玉米黄质进行分子对接。SMEAF对磷酸二酯酶-5（PDE-5）、精氨酸酶、血管紧张素I转换酶（ACE）、胆碱酯酶、单胺氧化酶A（MAO）、胞外5'核苷酸酶（E-NTDase）、酪氨酸酶具有显著的酶抑制作用，并刺激钠钾ATP酶（Na+/K+-ATPase）活性。HPLC/MS分析表明，酚类和类胡萝卜素是这些馏分中的主要成分，特别是绿原酸、叶黄素和玉米黄质。我们的结果表明，SMEAF可作为植物药物进行探索。实际应用：腰果树被作为具有巨大药用价值的绿色蔬菜烹饪，这可能归因于其具有强大抗氧化活性的多酚类物质。此外，其叶子对于治疗勃起功能障碍和中枢神经系统疾病也可能有用。
