Omojokun Olasunkanmi S, Famurewa Akindele J, Jaiyeoba Oluwademilade A, Oboh Ganiyu, Agbebi Oluwaseun J
Biochemistry Unit, Department of Physical & Chemical Sciences, Elizade University, Ilara-mokin, Nigeria.
Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, The Federal University of Technology, Akure, Nigeria.
J Food Biochem. 2019 Jun;43(6):e12889. doi: 10.1111/jfbc.12889. Epub 2019 May 15.
The erectogenic potential of alkaloids extracted from Bitter leaf (Vernonia amygdalina) and Black nightshade (Solanum nigrum) was investigated in this study. Fresh leaves obtained from Bitter leaf and Black night shade were air-dried, pulverized, and extracted for alkaloids. The inhibitory potential of the alkaloid extracts on arginase and phosphodiesterase-5 (PDE-5) activities in rats penile tissue was determined in vitro. The antioxidant properties were also evaluated and the constituent alkaloids quantified using GC-MS. The alkaloid extracts inhibited arginase (0-30.51 μg/ml) and PDE-5 (0-133.69 μg/ml) activities in a concentration-dependent pattern. Similarly, the alkaloid extracts inhibited Fe -induced lipid peroxidation in rats penile tissues, scavenged DPPH, OH, and NO radicals as a function of concentration. GC-MS characterization revealed over 20 alkaloid compounds. The inhibition of PDE-5-, arginase-, pro-oxidant-induced lipid peroxidative-, and free radicals-scavenging activities by the alkaloids is suggestive of putative mechanisms underlying their therapeutic use for managing erectile dysfunction in folklore medicine. PRACTICAL APPLICATIONS: Alkaloids extracted from Black nightshade (Solanum nigrum) and Bitter leaf (Vernonia amygdalina) were characterized and investigated by standard procedures for inhibitory action against key erectile dysfunction-linked enzymes and antioxidant activity. The alkaloids inhibited erectile dysfunction-linked enzymes (arginase and PDE-5) and showed considerable antioxidant activity in a concentration-dependent manner. In view of this, we suggest the application of these results in the development of erectile dysfunction drugs in the pharmaceutical industry, with probable minimal or no adverse effect.
本研究调查了从苦叶(扁桃斑鸠菊)和龙葵中提取的生物碱的勃起增强潜力。从苦叶和龙葵获取的新鲜叶子经风干、粉碎后提取生物碱。在体外测定生物碱提取物对大鼠阴茎组织中精氨酸酶和磷酸二酯酶-5(PDE-5)活性的抑制潜力。还评估了其抗氧化特性,并使用气相色谱-质谱联用仪对生物碱成分进行定量分析。生物碱提取物以浓度依赖性方式抑制精氨酸酶(0 - 30.51μg/ml)和PDE-5(0 - 133.69μg/ml)的活性。同样,生物碱提取物抑制大鼠阴茎组织中Fe诱导的脂质过氧化,清除DPPH、OH和NO自由基,且呈浓度依赖性。气相色谱-质谱联用仪表征显示有20多种生物碱化合物。生物碱对PDE-5、精氨酸酶、促氧化剂诱导的脂质过氧化和自由基清除活性的抑制作用提示了其在民间医学中用于治疗勃起功能障碍的潜在机制。实际应用:通过标准程序对从龙葵和苦叶中提取的生物碱进行表征和研究,以确定其对与勃起功能障碍相关的关键酶的抑制作用和抗氧化活性。这些生物碱抑制与勃起功能障碍相关的酶(精氨酸酶和PDE-5),并以浓度依赖性方式表现出相当的抗氧化活性。鉴于此,我们建议将这些结果应用于制药行业勃起功能障碍药物的研发,可能产生的副作用极小或无副作用。