Mchunu Nomcebo, Chukwuma Chika Ifeanyi, Ibrahim Mohammed Auwal, Oyebode Olajumoke A, Dlamini Siphiwe Ndumiso, Islam Md Shahidul
School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, South Africa.
Department of Pharmacology, University of the Free State, Bloemfontein, South Africa.
J Food Biochem. 2019 Mar;43(3):e12775. doi: 10.1111/jfbc.12775. Epub 2019 Jan 21.
Non-nutritive sweeteners (NNS) are increasingly being used by diabetics, but little is known about their effects on antioxidant status. We investigated the effects of ad libitum consumption of commercially available NNS (aspartame, saccharin, sucralose, and cyclamate-based sweeteners) on antioxidative markers in a rat model of type 2 diabetes (T2D). NNS consumption reduced (p < 0.05) T2D-induced lipid peroxidation and boosted serum, hepatic, renal, cardiac, and pancreatic glutathione (GSH) levels. Catalase, glutathione reductase, superoxide dismutase, and glutathione peroxidase activity was increased in the serum and most organs upon diabetes induction, perhaps due to adaptative antioxidant response to the diabetes-induced lipid peroxidation. NNS showed varying effects on serum and tissue antioxidant enzymes of animals. An antioxidant capacity scores sheet of NNS, suggest that aspartame-based NNS may not exert antioxidant effects in diabetics, while saccharin-based NNS may be a potent antioxidative sweetener as seen in the animal model of T2D. PRACTICAL APPLICATIONS: The use of NNS is becoming more popular, especially for diabetic individuals. While there are several commercial NNS available in the market, little is known about how they affect the antioxidant status of consumers. We therefore investigated how some commercially available NNS affect the antioxidant status of diabetic rats. Observed data revealed varying effects of NNS on serum and different organs, which suggest that some NNS may be better than others for diabetic oxidative stress and thus may be recommended for consumers. However, this finding is subject to additional corroborative clinical studies.
非营养性甜味剂（NNS）越来越多地被糖尿病患者使用，但人们对其对抗氧化状态的影响知之甚少。我们研究了随意食用市售NNS（阿斯巴甜、糖精、三氯蔗糖和甜蜜素类甜味剂）对2型糖尿病（T2D）大鼠模型抗氧化标志物的影响。食用NNS可降低（p < 0.05）T2D诱导的脂质过氧化，并提高血清、肝脏、肾脏、心脏和胰腺中的谷胱甘肽（GSH）水平。糖尿病诱导后，血清和大多数器官中的过氧化氢酶、谷胱甘肽还原酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性增加，这可能是由于对糖尿病诱导的脂质过氧化的适应性抗氧化反应。NNS对动物的血清和组织抗氧化酶有不同影响。NNS的抗氧化能力评分表表明，基于阿斯巴甜 的NNS可能不会对糖尿病患者发挥抗氧化作用，而基于糖精的NNS在T2D动物模型中可能是一种有效的抗氧化甜味剂。实际应用：NNS的使用越来越普遍，尤其是对于糖尿病患者。虽然市场上有几种商用NNS，但人们对它们如何影响消费者的抗氧化状态知之甚少。因此，我们研究了一些市售NNS如何影响糖尿病大鼠的抗氧化状态。观察到的数据显示NNS对血清和不同器官有不同影响，这表明对于糖尿病氧化应激，一些NNS可能比其他NNS更好，因此可能推荐给消费者。然而，这一发现有待进一步的临床对照研究证实。
