Oboh Ganiyu, Adebayo Adeniyi A, Ademosun Ayokunle O
Functional Foods and Nutraceutical Research Unit, Department of Biochemistry, Federal University of Technology, P.M.B. 704, Akure, Nigeria, Phone: +2347031388644.
Functional Foods and Nutraceutical Research Unit, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
J Basic Clin Physiol Pharmacol. 2018 Nov 27;29(6):689-696. doi: 10.1515/jbcpp-2017-0160.
Herbs have been used from ages to manage male sexual dysfunction. Hence, this study sought to investigate the effects of Eurycoma longifolia (EL) and Cylicodiscus gabunensis (CG) stem bark extracts on some enzymes implicated in erectile dysfunction in vitro.
The extracts were prepared, and their effects on phosphodiesterase-5 (PDE-5), arginase, and angiotensin-1-converting enzyme (ACE) as well as pro-oxidant-induced lipid peroxidation were assessed. Furthermore, phenolic contents were determined, and their components were characterized and quantified using high-performance liquid chromatography with diode array detector (HPLC-DAD).
The results revealed that the extracts inhibited PDE-5, arginase, and ACE in a concentration-dependent manner. However, IC50 values revealed that CG had higher inhibitory potential on PDE-5 (IC50=204.4 μg/mL), arginase (IC50=39.01 μg/mL), and ACE (IC50=48.81 μg/mL) than EL. In addition, the extracts inhibited pro-oxidant-induced lipid peroxidation in penile tissue homogenate. HPLC-DAD analysis showed that CG is richer in phenolic compounds than EL, and this could be responsible for higher biological activities observed in CG than EL.
Hence, the observed antioxidant property and inhibitory action of CG and EL on enzymes relevant to erectile dysfunction in vitro could be part of possible mechanisms underlying their involvement in traditional medicine for the management of male sexual dysfunction.
草药长期以来一直被用于治疗男性性功能障碍。因此，本研究旨在调查长叶刺蒺藜（EL）和加蓬旋果豆（CG）茎皮提取物对体外勃起功能障碍相关一些酶的影响。
制备提取物，并评估其对磷酸二酯酶-5（PDE-5）、精氨酸酶和血管紧张素-1转换酶（ACE）的影响以及促氧化剂诱导的脂质过氧化作用。此外，测定酚类含量，并使用带二极管阵列检测器的高效液相色谱法（HPLC-DAD）对其成分进行表征和定量。
结果显示，提取物以浓度依赖性方式抑制PDE-5、精氨酸酶和ACE。然而，半数抑制浓度（IC50）值表明，CG对PDE-5（IC50=204.4μg/mL）、精氨酸酶（IC50=39.01μg/mL）和ACE（IC50=48.81μg/mL）的抑制潜力高于EL。此外，提取物抑制阴茎组织匀浆中促氧化剂诱导的脂质过氧化。HPLC-DAD分析表明，CG中的酚类化合物比EL更丰富，这可能是CG比EL具有更高生物活性的原因。
因此，观察到的CG和EL的抗氧化特性以及对体外勃起功能障碍相关酶的抑制作用，可能是它们参与传统医学治疗男性性功能障碍潜在机制的一部分。
