Yu Chunshui, Liu Linli, Liao Qian, Wang Hanwen, Ouyang Fei, Fang Hui, Wan Yan, Lu Qinlian, Liu Tingting
Department of Dermatology, Suining Central Hospital, No. 127, Western Desheng Road, Suining, 629000 China.
3 Biotech. 2019 Aug;9(8):301. doi: 10.1007/s13205-019-1828-z. Epub 2019 Jul 22.
The aim of this study was to explore the anti-psoriatic effect and potential mechanism of Angelica polysaccharide (APS) on an in vitro HaCaT cell model. MTS assay was performed to determine whether APS has the ability to inhibit the proliferation of HaCaT cells. RNA-sequencing (RNA-seq) was performed to investigate the underlying mechanism of APS. Quantitative real-time PCR (qRT-PCR) was used to verify the accuracy of RNA-seq data. Our MTS assay results demonstrated that APS time- and concentration-dependently inhibits the proliferation of HaCaT cells. The anti-proliferation property of APS suggests that APS may have anti-psoriatic effect. In the RNA-seq part, comparison between the CK group (i.e., Control group) and ASP groups revealed dramatic differences [468 differentially expressed genes (DEGs) for CK group vs. ASP50 group; 563 DEGs for CK group vs. ASP100 group; 532 DEGs for CK group vs. ASP200 group]. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrich analysis performed on all DEGs failed to find any significant enriched GO terms or KEGG pathways to explain the anti-proliferative effect of APS. All DEGs were then classified into 20 expression profiles by trend analysis. Interestingly, cell proliferation-related GO terms were mostly dispersed in the profile 2 and 17. DEGs enriched in these terms were then analyzed. After literature retrieval, DEGs such as , , , and - were suspected to closely relevant to the anti-proliferation effect of APS. qRT-PCR results showed similar expression trend with RNA-seq data for 8 out of 10 genes, indicating our sequence data are reliable.
本研究旨在探讨当归多糖（APS）在体外HaCaT细胞模型上的抗银屑病作用及潜在机制。采用MTS法检测APS是否具有抑制HaCaT细胞增殖的能力。进行RNA测序（RNA-seq）以研究APS的潜在机制。采用定量实时PCR（qRT-PCR）验证RNA-seq数据的准确性。我们的MTS分析结果表明，APS对HaCaT细胞增殖的抑制作用具有时间和浓度依赖性。APS的抗增殖特性表明其可能具有抗银屑病作用。在RNA-seq部分，CK组（即对照组）与ASP组之间的比较显示出显著差异[CK组与ASP50组有468个差异表达基因（DEG）；CK组与ASP100组有563个DEG；CK组与ASP200组有532个DEG]。对所有DEG进行的基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析未能找到任何显著富集的GO术语或KEGG通路来解释APS的抗增殖作用。然后通过趋势分析将所有DEG分为20个表达谱。有趣的是，细胞增殖相关的GO术语大多分散在谱2和谱17中。然后对富集在这些术语中的DEG进行分析。经过文献检索，怀疑如 、 、 和 - 等DEG与APS的抗增殖作用密切相关。qRT-PCR结果显示，10个基因中有8个基因的表达趋势与RNA-seq数据相似，表明我们的序列数据可靠。
