Department of Specialization in Periodontology, Faculty of Dentistry, Autonomous University of Yucatan, Merida, Yucatan, ZIP 97000, Mexico.
Cellular Biology Laboratory, Regional Research Center "Dr. Hideyo Noguchi", University of Yucatan, Merida, Yucatan, ZIP 97000, Mexico.
Biomed Res Int. 2019 Jul 2;2019:5613514. doi: 10.1155/2019/5613514. eCollection 2019.
The present study aimed to compare levels of matrix metalloproteinase-9 (MMP-9) and myeloperoxidase (MPO) in gingival crevicular fluid (GCF) from subjects with controlled and noncontrolled Type 2 Diabetes Mellitus (T2D), with and without stage 2 grade B periodontitis (POD2B) versus healthy (H) subjects.
The levels of both enzymes, from 80 GCF samples collected with PerioPaper strips, were analyzed by a Multiplex/Luminex assay. Five groups were formed, all current patients at the Institutional Dentistry Service, and distributed as follows: two groups of diabetics (one controlled and one poorly controlled); two groups with the previous conditions and diagnosed with POD2B; and one H group.
The highest concentration of MMP-9 corresponded to the H group, while the lowest corresponded to the T2D controlled group. Regarding MPO levels, the highest levels were associated with the T2D controlled with POD2B group and the lowest with the T2D controlled group.
No apparent relationship between the elevation of MMP-9 and MPO levels was observed among subjects with T2D, with and without POD2B, compared to H subjects.
本研究旨在比较伴有 2 型糖尿病（T2DM）且控制良好和控制不佳、伴或不伴 2 级 B 期牙周炎（POD2B）与健康（H）受试者龈沟液（GCF）中基质金属蛋白酶-9（MMP-9）和髓过氧化物酶（MPO）水平。
使用 PerioPaper 条采集 80 份 GCF 样本，通过多重 Luminex 检测分析两种酶的水平。所有受试者均为机构牙科服务处的现患病人，分为以下五组：两组糖尿病患者（一组控制良好，一组控制不佳）；两组具有上述情况并诊断为 POD2B；一组 H 组。
MMP-9 浓度最高的为 H 组，最低的为 T2D 控制组。关于 MPO 水平，T2D 控制伴 POD2B 组的水平最高，T2D 控制组的水平最低。
与 H 组相比，伴有和不伴有 POD2B 的 T2D 患者的 MMP-9 和 MPO 水平升高之间似乎没有明显关系。
