State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau.
Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.
Biomed Res Int. 2019 Jul 1;2019:7465272. doi: 10.1155/2019/7465272. eCollection 2019.
In parallel with the prevalence metabolic syndrome, nonalcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease in most countries. It features a constellation of simple steatosis, nonalcoholic steatohepatitis (NASH), fibrosis, cirrhosis, and even hepatocellular carcinoma. There are no approved drugs for effective management of NAFLD and NASH. Jianpi Huoxue formula (JPHX) mainly consists of Atractylodes macrocephal , Salvia miltiorrhiza (), Rasux Paeonia Alba (), Rhizoma Alismatis (), and Fructus Schisandrae Chinensis (), which may have beneficial effects on NAFLD. The aim of the study was to identify the effect of JPHX on NAFLD. A NAFLD model was induced by methionine-choline-deficient food (MCD) in Wistar rats and orally administered with simultaneous JPHX, once a day for 8 weeks. Hepatocellular injury, lipid profile, inflammation, fibrosis, and apoptosis were evaluated. The results showed that JPHX significantly decreased the abnormal serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared with the MCD model (P<0.05). Furthermore, JPHX protected MCD diet-fed rats from accumulation of hepatic triglycerides (TG) and total cholesterol (TC). Histological examination demonstrated that JPHX noticeably normalized the NAFLD activity score (NAS). Moreover, JPHX ameliorated liver inflammation by decreasing TNF- levels and reduced collagen and matrix metalloproteinases in MCD diet-fed rats. In addition, JPHX prevented rats from MCD-induced cellular apoptosis, as suggested by TUNEL staining, and suppressed the activation of caspase 3 and 7 proteins. JPHX also inhibited the phosphorylation of JNK. In conclusion, JPHX exhibited a hepatoprotective effect against NAFLD in an MCD experimental model.
与代谢综合征的流行并行的是，非酒精性脂肪性肝病（NAFLD）已成为大多数国家最常见的慢性肝病。它的特征是一系列简单的脂肪变性、非酒精性脂肪性肝炎（NASH）、纤维化、肝硬化，甚至肝细胞癌。目前尚无批准用于有效管理 NAFLD 和 NASH 的药物。健脾活血方（JPHX）主要由白术、丹参（）、白芍（）、泽泻（）和五味子（）组成，可能对 NAFLD 有有益的作用。本研究旨在确定 JPHX 对 NAFLD 的影响。采用蛋氨酸-胆碱缺乏饲料（MCD）诱导 Wistar 大鼠 NAFLD 模型，同时口服 JPHX，每天 1 次，连续 8 周。评估肝细胞损伤、血脂谱、炎症、纤维化和细胞凋亡。结果表明，与 MCD 模型相比，JPHX 显著降低了异常血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平（P<0.05）。此外，JPHX 保护 MCD 饮食喂养大鼠免受肝甘油三酯（TG）和总胆固醇（TC）的积累。组织学检查表明，JPHX 明显使 NAFLD 活动评分（NAS）正常化。此外，JPHX 通过降低 TNF-水平和减少 MCD 饮食喂养大鼠的胶原和基质金属蛋白酶来改善肝脏炎症。此外，JPHX 可防止 MCD 诱导的细胞凋亡，如 TUNEL 染色所示，并抑制 caspase 3 和 7 蛋白的激活。JPHX 还抑制了 JNK 的磷酸化。总之，JPHX 在 MCD 实验模型中表现出对 NAFLD 的肝保护作用。
