Wang Cong, Qiu Zi-Yan, Lei Fu-Hou, Tan Xue-Cai, Jiang Ming-Guo, Li Hao, Li Xiao-Yan
Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources,School of Marine Sciences and Biotechnology,Guangxi University for Nationalities Nanning 530006,China Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities Nanning 530006,China.
Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities Nanning 530006,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2090-2095. doi: 10.19540/j.cnki.cjcmm.20190222.010.
To isolate and identify secondary metabolites of marine-derived Streptomyces sp.MDW-06,the isolations and purifications of compounds were performed by means of column chromatography over silica gel. And their structures were elucidated through the spectroscopic analysis of MS,NMR and specific rotations. The bioactivities were assayed by paper diffusion and DPPH method. From the fermentation broth of marine-derived Streptomyces sp.MDW-06,five compounds( 1-5) were isolated and identified as streptopentanoic acid( 1),germicidin A( 2),germicidin B( 3),isogermicidin A( 4),isogermicidin B( 5) and oxohygrolidin( 6),respectively. Compound 1 is a new compound. Compound 1 shows DPPH radical scavenging activity with 36. 4% at 100 mg·L~(-1).
为了分离和鉴定海洋来源的链霉菌属菌株MDW-06的次生代谢产物，采用硅胶柱色谱法进行化合物的分离和纯化。并通过质谱、核磁共振和比旋光度的光谱分析来阐明其结构。采用纸片扩散法和DPPH法测定生物活性。从海洋来源的链霉菌属菌株MDW-06的发酵液中，分离并鉴定出5种化合物（1-5），分别为链戊酸（1）、杀菌素A（2）、杀菌素B（3）、异杀菌素A（4）、异杀菌素B（5）和氧代 Hygrolidin（6）。化合物1是一种新化合物。化合物1在100 mg·L⁻¹ 时具有36.4% 的DPPH自由基清除活性。
