Zhou Li-Shan, Yan Su-Qi, Xiong Xiao-Li, Huang Juan, Qin Huan, Zhang Ling-Ling, Zhang Lin-Li, Tang Jian-Qiao
Wuhan Children's Hospital ( Wuhan Maternal and Child Healthcare Hospital) ,Tongji Medical College,Huazhong University of Science & Technology Wuhan 430016,China.
Integrated Traditional Chinese and Western Medicine Hospital of Wuhan Wuhan 430022,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2118-2123. doi: 10.19540/j.cnki.cjcmm.20190129.004.
The aim of this paper was to investigate the mechanism and effect of psoralen and isopsoralen in the treatment of lipid accumulation in LO2 cells. Human LO2 cells nonalcoholic fatty liver models were established by using palmitic acid( PA). Then psoralen and isopsoralen were administered for intervention. Intracellular triglyceride( TG) and total cholesterol( TC) content,the cell supernatant alanine aminotransferase( ALT) and aspartate aminotransferase( AST) levels were determined by enzyme method. Cell supernatant proinflammatory cytokines( IL-6,TNF-α) and chemokines( IL-8,MCP-1) were determined by ELISA method. Western blot method was conducted to detect the protein expression of intracellular nuclear factor( NF-κB) p65 phosphorylation( p-p65),nonphosphorylated protein( p65),and transforming factor TGF-β1. Result showed that as compared with the model group,intracellular TG and TC levels,the cell supernatant ALT and AST levels,proinflammatory cytokines and chemokines were decreased( P < 0. 01,P <0. 05); the p-p65/p65 ratio and TGF-β1 protein expression were also significantly decreased( P< 0. 01,P< 0. 05) in psoralen intervention group. As compared with the model cells,intracellular TG content had no significant changes,but all the other indexes were reduced( P<0. 01,P<0. 05) in the cells of isopsoralen intervention group. Psoralen exhibited better effect than isopsoralen( P< 0. 01,P<0. 05). It is concluded that psoralen could improve the adipogenesis of LO2 cells induced by PA; both psoralen and isopsoralen are effective in ameliorating LO2 cells injury induced by PA,reducing inflammation via inhibiting the activation of NF-κB and down-regulating the expression of TGF-β1.
本文旨在探讨补骨脂素和异补骨脂素治疗LO2细胞脂质蓄积的机制及效果。采用棕榈酸（PA）建立人LO2细胞非酒精性脂肪肝模型，然后给予补骨脂素和异补骨脂素进行干预。采用酶法测定细胞内甘油三酯（TG）和总胆固醇（TC）含量、细胞上清液丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平；采用ELISA法测定细胞上清液促炎细胞因子（IL-6、TNF-α）和趋化因子（IL-8、MCP-1）；采用蛋白质免疫印迹法检测细胞内核因子（NF-κB）p65磷酸化（p-p65）、非磷酸化蛋白（p65）及转化生长因子β1（TGF-β1）的蛋白表达。结果显示，与模型组比较，补骨脂素干预组细胞内TG和TC水平、细胞上清液ALT和AST水平、促炎细胞因子和趋化因子均降低（P<0.01，P<0.05）；p-p65/p65比值及TGF-β1蛋白表达也显著降低（P<0.01，P<0.05）。与模型细胞比较，异补骨脂素干预组细胞内TG含量无显著变化，但其他各项指标均降低（P<0.01，P<0.05）。补骨脂素效果优于异补骨脂素（P<0.01，P<0.05）。结论：补骨脂素可改善PA诱导的LO2细胞脂肪生成；补骨脂素和异补骨脂素均能有效减轻PA诱导的LO2细胞损伤，通过抑制NF-κB激活、下调TGF-β1表达减轻炎症反应。
