Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, 200011, China.
Sci Rep. 2021 Apr 22;11(1):8754. doi: 10.1038/s41598-021-85145-1.
Psoralen is one of the most effective ingredients extracted from the Chinese herb, Psoralea corylifolia L. Studies have found that psoralen has anti-inflammatory and estrogen-like effects; however, little research has been conducted to elucidate the mechanisms underlying these effects. Through the molecule docking assay, psoralen was found to have a better combination with ERα than ERβ. In human periodontal ligament cells, psoralen was found to upregulate the estrogen target genes (e.g., CTSD, PGR, TFF1) and down-regulate the expression of inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-8) stimulated by P. gingivalis LPS, as well as TLR4-IRAK4-NF-κb signaling pathway proteins. These effects were reversed by the ER antagonist ICI 182780. These results indicated that psoralen may exert anti-inflammatory effects as an agonist to ER, which could provide a theoretical basis for the use of psoralen for adjuvant therapy and prevention of periodontitis.
补骨脂素是从中药补骨脂中提取的最有效的成分之一。研究发现，补骨脂素具有抗炎和类雌激素作用；然而，对于这些作用的机制研究甚少。通过分子对接试验，发现补骨脂素与 ERα 的结合优于 ERβ。在人牙周韧带细胞中，补骨脂素可上调雌激素靶基因（如 CTSD、PGR、TFF1），并下调 P. gingivalis LPS 刺激的炎症细胞因子（TNF-α、IL-1β、IL-6 和 IL-8）的表达，同时下调 TLR4-IRAK4-NF-κb 信号通路蛋白。这些作用可被 ER 拮抗剂 ICI 182780 逆转。这些结果表明，补骨脂素可能作为 ER 的激动剂发挥抗炎作用，这可为补骨脂素用于辅助治疗和预防牙周炎提供理论依据。
