sp. nov., an actinobacterium isolated from the rhizosphere soil of wheat ( L.).

A Gram-stain-positive, aerobic, heterotrophic, non-spore-forming and rod-shaped strain, designated SJ-23, was isolated from rhizosphere soil of wheat ( L.) collected from Langfang, Hebei Province, central PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid and three unidentified lipids. The predominant menaquinones detected were MK-12, MK-11 and MK-10. Major fatty acids were identified as anteiso-C, anteiso-C and iso-C. The 16S rRNA gene sequence analysis showed that strain SJ-23 belongs to the genus with high sequence similarities to DSM 43045 (99.2 %), subsp. DSM 8595 (98.8 %) and subsp. DSM 8596 (98.6 %). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a separate branch in the genus . Furthermore, the combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-23 could be distinguished from its closest relatives. Therefore, it is proposed that strain SJ-23 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SJ-23 (=CGMCC 4.7419=DSM 105049).