Duan Liping, Song Wei, Jiang Shanwen, Qian Lulu, Guo Xiaowei, Wang Xiangjing, Zhao Junwei, Xiang Wensheng
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 May;112(5):703-710. doi: 10.1007/s10482-018-1200-z. Epub 2018 Nov 21.
Three Gram-stain positive, aerobic actinomycete strains, designated NEAU-A15, NEAU-A13 and NEAU-C4, were isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Langfang, Hebei Province, China. Based on their morphological characteristics, biochemical features and molecular phylogenetic studies, these strains were concluded to belong to a new member of the genus Glycomyces. 16S rRNA gene sequence analysis showed that strain NEAU-A15 shares 99.91% and 99.80% 16S rRNA gene sequence similarity with NEAU-A13 and NEAU-C4, respectively, and these three strains showed high sequence similarities to Glycomyces algeriensis DSM 44727 (99.24, 99.45, 99.38%), Glycomyces lechevalierae DSM 44724 (98.97, 99.17, 99.11%) and Glycomyces rutgersensis DSM 43812 (98.83, 99.04, 98.97%). Phylogenetic analysis indicated that these three strains clustered together and formed a cluster with Glycomyces tritici NEAU-C2 (97.30, 97.73, 99.48%), G. algeriensis DSM 44727, G. lechevalierae DSM 44724 and G. rutgersensis DSM 43812. These three strains were observed to contain MK-10(H), MK-10(H) and MK-11 as menaquinones. The whole cell sugar profiles were found to contain galactose, ribose and xylose. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipid, phosphatidylinositol mannoside and an unidentified glycolipid. The major fatty acids were identified as anteiso-C, iso-C, anteiso-C and iso-C. The DNA-DNA hybridization values between strain NEAU-A15 and NEAU-A13/NEAU-C4 were 86.2 ± 2.3% and 83.4 ± 3.5%, respectively. The values between these three strains and their close phylogenetic relatives were 48-52%, supporting the conclusion that they belong to a distinct genomic species. An array of phenotypic characteristics also differentiated these isolates from their closely related species. On the basis of the genetic and phenotypic properties, strains NEAU-A15, NEAU-A13 and NEAU-C4 can be classified as representatives of a novel species of the genus Glycomyces, for which the name Glycomyces luteolus sp. nov., is proposed. The type strain is NEAU-A15 (= DSM 104643 = CGMCC 4.7394).
从中国河北省廊坊市采集的小麦（Triticum aestivum L.）根际土壤中分离出三株革兰氏染色阳性的需氧放线菌，分别命名为NEAU-A15、NEAU-A13和NEAU-C4。基于它们的形态特征、生化特性和分子系统发育研究，这些菌株被认定为糖霉菌属的一个新成员。16S rRNA基因序列分析表明，菌株NEAU-A15与NEAU-A13和NEAU-C4的16S rRNA基因序列相似性分别为99.91%和99.80%，这三株菌株与阿尔及利亚糖霉菌DSM 44727（99.24%、99.45%、99.38%）、勒氏糖霉菌DSM 44724（98.97%、99.17%、99.11%）和罗格斯糖霉菌DSM 43812（98.83%、99.04%、98.97%）显示出较高的序列相似性。系统发育分析表明，这三株菌株聚集在一起，并与小麦糖霉菌NEAU-C2（97.30%、97.73%、99.48%）、阿尔及利亚糖霉菌DSM 44727、勒氏糖霉菌DSM 44724和罗格斯糖霉菌DSM 43812形成一个聚类。观察到这三株菌株含有MK-10(H)、MK-10(H)和MK-11作为甲基萘醌。发现全细胞糖谱包含半乳糖、核糖和木糖。发现极性脂质由双磷脂酰甘油、磷脂酰肌醇、磷脂酰甘油、磷酸糖脂、磷脂酰肌醇甘露糖苷和一种未鉴定的糖脂组成。主要脂肪酸被鉴定为anteiso-C、iso-C、anteiso-C和iso-C。菌株NEAU-A15与NEAU-A13/NEAU-C4之间的DNA-DNA杂交值分别为86.2±2.3%和83.4±3.5%。这三株菌株与其近缘系统发育亲属之间的值为48-52%，支持它们属于一个独特基因组物种的结论。一系列表型特征也使这些分离株与其近缘物种区分开来。基于遗传和表型特性，菌株NEAU-A15、NEAU-A13和NEAU-C4可被归类为糖霉菌属一个新物种的代表，为此提出新名称黄褐糖霉菌（Glycomyces luteolus sp. nov.）。模式菌株是NEAU-A15（=DSM 104643 = CGMCC 4.7394）。
