Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY, USA; Department of Biomedical Sciences, Long Island University-Post, Brookville, NY, USA.
Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, New York Institute of Technology, Old Westbury, NY, USA.
Fungal Genet Biol. 2019 Nov;132:103258. doi: 10.1016/j.fgb.2019.103258. Epub 2019 Jul 26.
Cryptococcus neoformans is an opportunistic encapsulated pathogen that causes life-threatening meningoencephalitis in individuals with immunosuppression. We compared the interactions of C. neoformans planktonic and biofilm-derived cells with J774.16 macrophage-like cells. Planktonic cells are more phagocytized and killed by J774.16 cells than biofilm-derived fungal cells. Biofilm-derived cryptococci possess larger capsule size and release significantly more capsular polysaccharide than planktonic cells in culture. Biofilm-derived fungi exhibited upregulation of genes involved in capsular production. Capsular-specific monoclonal antibody 18B7 demonstrated differential binding to the surface of planktonic and biofilm-derived cryptococci providing a plausible strategy for fungal evasion of macrophages and persistence. Future studies are necessary to elucidate how C. neoformans biofilm-derived cells regulate their virulence factors when interacting with cells of the immune system.
新型隐球菌是一种机会性囊胞病原体，可导致免疫抑制个体发生致命性脑膜脑炎。我们比较了新型隐球菌浮游和生物膜衍生细胞与 J774.16 巨噬样细胞的相互作用。浮游细胞比生物膜衍生的真菌细胞更容易被 J774.16 细胞吞噬和杀死。生物膜衍生的隐球菌在培养中具有更大的荚膜大小，并释放出比浮游细胞显著更多的荚膜多糖。生物膜衍生的真菌表现出与荚膜产生相关的基因上调。荚膜特异性单克隆抗体 18B7 显示出对浮游和生物膜衍生隐球菌表面的差异结合，为真菌逃避巨噬细胞和持续存在提供了一种合理的策略。未来的研究有必要阐明新型隐球菌生物膜衍生细胞在与免疫系统细胞相互作用时如何调节其毒力因子。
