Université de Lorraine, CNRS, LCPM, F-5400 Nancy, France; Chemistry Department, Faculty of Science, Cairo University, 12613 Giza, Egypt.
Université de Lorraine, CNRS, LCPM, F-5400 Nancy, France.
Colloids Surf B Biointerfaces. 2019 Oct 1;182:110393. doi: 10.1016/j.colsurfb.2019.110393. Epub 2019 Jul 24.
In this work, photo-sensitive core/shell nanoparticles (NPs) based on biocompatible dextran-g-poly(o-nitrobenzyl acrylate) copolymers (Dex-g-PNBA), containing dextran as hydrophilic backbone and PNBA as photosensitive grafts, were formulated using two processes. In the first process (nanoprecipitation), NPs were prepared using preformed Dex-g-PNBA copolymers. Using the second process (emulsion/organic solvent evaporation), "clicked" or "unclicked" NPs were obtained carrying out (or not) an interfacial in situ click chemistry, respectively. Two model molecules, Nile Red (NR) and Doxorubicin (DOX), were encapsulated and their controlled release from NPs was investigated under UV irradiations to demonstrate the high potential of such photosensitive NPs in biomedicine applications as drug delivery nanocarriers. According to such irradiations, improved release was easily observed. Release kinetics depended on the formulation process and the NPs core chemistry, but not on the occurrence of the interfacial in situ click chemistry. More interesting, a stepped release of such model molecules may easily be obtained.
在这项工作中，基于生物相容性葡聚糖-g-聚（邻硝基苄基丙烯酰胺）共聚物（Dex-g-PNBA）的光敏核/壳纳米粒子（NPs）被制定了两种方案。在第一种方案（纳米沉淀）中，使用预先制备的 Dex-g-PNBA 共聚物来制备 NPs。使用第二种方案（乳液/有机溶剂蒸发），通过界面原位点击化学（或不进行），分别得到“点击”或“未点击”的 NPs。封装了两种模型分子尼罗红（NR）和阿霉素（DOX），并在 UV 照射下研究了它们从 NPs 中的控制释放，以证明这种光敏 NPs 在药物输送纳米载体等生物医学应用中的巨大潜力。根据这些照射，很容易观察到释放的改善。释放动力学取决于配方工艺和 NPs 核化学，而与界面原位点击化学的发生无关。更有趣的是，可以很容易地获得这种模型分子的分步释放。
