National Applied Research Laboratories,Taiwan Semiconductor Research Institute, Hsinchu, Taiwan; Department of Materials Science and Engineering, National Tsing Hua University, Hsinchu, Taiwan.
National Applied Research Laboratories,Taiwan Semiconductor Research Institute, Hsinchu, Taiwan; Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Biosens Bioelectron. 2019 Sep 15;141:111209. doi: 10.1016/j.bios.2019.03.042. Epub 2019 Mar 21.
Enhancing the efficiency of antibody protein immobilized on a silicon nanowire-based chip for their antigens detection is reported. An external electric field (EEF) is applied to direct the orientation of antibodies during their immobilization on a chip. Atomic force microscopy (AFM) is used to measure the binding forces between immobilized antibody and targeting antigen under the influence of EEF at different angles. The maximum binding force under a specific angle (optimal angle; oa) of EEF (EEF) implies the optimal orientation of the antibodies on the chip. In this report, two different cancer carcinoembryonic antigen (CEA)-related cell adhesion molecules 5 (CEACAM5) & 1 (CEACAM1) were used for the examples of disease antigen detection. EEF of anti-CEACAM5 or anti-CEACAM1 immobilized on a general chip was firstly determined. Spectroscopy of AFM revealed that both binding forces were the largest ones with their antigens when EEF was applied as compared with no or other angles of EEF. These antibody proteins accompanied with the application of EEF were secondly immobilized on silicon-nanowires (n = 1000) and the field effects were measured (∆I) as their target antigens were approached. Results showed that ∆I was the largest ones when EEFs (225°/270° and 135°/180° for anti-CEACAM5 and anti-CEACAM1, respectively) were applied as compared with other angles of EEF. These observations imply that the silicon nanowires together with the application of EEF as detection tools could be applied for the cancer diagnostics in the future.
报道了一种提高硅纳米线芯片上固定的抗体蛋白与其抗原检测效率的方法。在抗体固定在芯片上的过程中，施加外部电场（EEF）以引导抗体的取向。原子力显微镜（AFM）用于测量在不同角度的 EEF 影响下固定化抗体与靶向抗原之间的结合力。在特定 EEF 角度（最佳角度；oa）下的最大结合力（EEF）意味着抗体在芯片上的最佳取向。在本报告中，使用两种不同的癌胚抗原（CEA）相关细胞粘附分子 5（CEACAM5）和 1（CEACAM1）作为疾病抗原检测的示例。首先确定了固定在普通芯片上的抗-CEACAM5 或抗-CEACAM1 的 EEF。与没有 EEF 或其他角度的 EEF 相比，AFM 光谱显示，当施加 EEF 时，这两种结合力与它们的抗原都是最大的。这些抗体蛋白伴随着 EEF 的应用，其次被固定在硅纳米线（n=1000）上，并测量了它们的目标抗原接近时的场效应（∆I）。结果表明，与其他 EEF 角度相比，当应用 EEF（分别为抗-CEACAM5 和抗-CEACAM1 的 225°/270°和 135°/180°）时，∆I 最大。这些观察结果表明，硅纳米线与 EEF 的应用相结合，可以作为未来癌症诊断的检测工具。
