Chakraborty Chumki, Thompson S, Lyons Veronica J, Snoeyink Craig, Pappas Dimitri
Department of Mechanical Engineering, Texas Tech University, Lubbock, TX 79409, United States of America.
Nanotechnology. 2019 Nov 8;30(45):455704. doi: 10.1088/1361-6528/ab368d. Epub 2019 Jul 29.
Blinking of fluorescent nanoparticles is a compelling phenomenon with widely debated mechanisms. The ability to inhibit or control blinking is important for applications in the field of optical, semiconductor and fluorescent imaging. Self-blinking nanomaterials are also attractive labels for localization-based super-resolution microscopy. In this work, we have synthesized silver core silica nanoparticles (Ag@SiO) doped with Rhodamine 110 and studied the parameters that affect blinking. We found that under nitrogen rich conditions the nanoparticles shifted towards higher duty cycles. Also, it was found that hydrated nanoparticles showed a less drastic response to nitrogen rich conditions as compared to dried nanoparticles, indicating that surrounding matrix played a role in the response of nanoparticles to molecular oxygen. Further, the blinking is not a multi-body phenomena, super-resolution localization combined with intensity histogram analysis confirmed that single particles are emitting.
荧光纳米颗粒的闪烁是一种引人注目的现象，其机制存在广泛争议。抑制或控制闪烁的能力对于光学、半导体和荧光成像领域的应用至关重要。自闪烁纳米材料也是基于定位的超分辨率显微镜的有吸引力的标记物。在这项工作中，我们合成了掺杂若丹明110的银核二氧化硅纳米颗粒（Ag@SiO），并研究了影响闪烁的参数。我们发现，在富氮条件下，纳米颗粒的占空比向更高值偏移。此外，还发现与干燥的纳米颗粒相比，水合纳米颗粒对富氮条件的响应不那么剧烈，这表明周围基质在纳米颗粒对分子氧的响应中起作用。此外，闪烁不是多体现象，超分辨率定位结合强度直方图分析证实单个颗粒在发光。
