Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Talanta. 2019 Nov 1;204:685-692. doi: 10.1016/j.talanta.2019.06.031. Epub 2019 Jun 15.
With the development of flexible advanced materials and microfluidic technology, wearable biosensors provide a new strategy for the continuous monitoring of health. In this study, a novel bandage-like wearable flexible microfluidic recombinase polymerase amplification (RPA) sensor was constructed for the rapid and visual detection of nucleic acids. This wearable sensor is triggered by human body heat (30°C-37 °C) and allows for visual nucleic acid (a conserved nucleic acid fragments of zika virus) detection within 10 min. The sensor displays good sensitivity and selectivity, with a detection limit of 10 copies/μL. The wearable sensor has exhibited well-defined accuracy when applied to testing clinical serum samples. In addition, the wearable RPA sensor was proved to be feasible by human trials under different daily activities. This wearable sensor of nucleic acids will probably be of great significance in the field of online pathogen detection for wounds, for tumour biomarker diagnosis, and for the detection of epidermal cell molecular lesions.
随着柔性先进材料和微流控技术的发展，可穿戴生物传感器为健康的连续监测提供了新策略。在这项研究中，构建了一种新颖的绷带式可穿戴柔性微流控重组酶聚合酶扩增（RPA）传感器，用于快速可视化检测核酸。这种可穿戴传感器由人体热量（30°C-37°C）触发，可在 10 分钟内实现可视化核酸（寨卡病毒的保守核酸片段）检测。该传感器具有良好的灵敏度和选择性，检测限为 10 拷贝/μL。该可穿戴传感器在检测临床血清样本时表现出良好的准确性。此外，该可穿戴 RPA 传感器在不同日常活动下的人体试验中被证明是可行的。这种用于检测伤口病原体、肿瘤生物标志物诊断以及表皮细胞分子病变的核酸可穿戴传感器具有重要意义。
