Department of Biomedical Engineering, University of Calgary, Calgary, AB T2N 1N4, Canada.
Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.
Biosensors (Basel). 2022 Nov 8;12(11):986. doi: 10.3390/bios12110986.
Wearable biosensors are becoming increasingly popular due to the rise in demand for non-invasive, real-time monitoring of health and personalized medicine. Traditionally, wearable biosensors have explored protein-based enzymatic and affinity-based detection strategies. However, in the past decade, with the success of nucleic acid-based point-of-care diagnostics, a paradigm shift has been observed in integrating nucleic acid-based assays into wearable sensors, offering better stability, enhanced analytical performance, and better clinical applicability. This narrative review builds upon the current state and advances in utilizing nucleic acid-based assays, including oligonucleotides, nucleic acid, aptamers, and CRISPR-Cas, in wearable biosensing. The review also discusses the three fundamental blocks, i.e., fabrication requirements, biomolecule integration, and transduction mechanism, for creating nucleic acid integrated wearable biosensors.
由于对非侵入性、实时健康监测和个性化医疗的需求不断增加，可穿戴生物传感器越来越受欢迎。传统上，可穿戴生物传感器探索了基于蛋白质的酶和基于亲和力的检测策略。然而，在过去十年中，随着基于核酸的即时诊断的成功，人们观察到将基于核酸的分析整合到可穿戴传感器中的范式转变，从而提供了更好的稳定性、增强的分析性能和更好的临床适用性。本综述建立在当前基于核酸的分析，包括寡核苷酸、核酸、适体和 CRISPR-Cas，在可穿戴生物传感中的应用的基础上。该综述还讨论了创建整合核酸的可穿戴生物传感器的三个基本模块，即制造要求、生物分子整合和转导机制。
