一种新型线粒体靶向近红外（NIR）探针，用于检测活细胞、斑马鱼和活体小鼠中粘度的变化。

A novel mitochondria-targeted near-infrared (NIR) probe for detection of viscosity changes in living cell, zebra fishes and living mice.

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, People's Republic of China; School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.

Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong, 250022, PR China.

出版信息

Talanta. 2019 Nov 1;204:868-874. doi: 10.1016/j.talanta.2019.06.050. Epub 2019 Jun 15.

DOI:10.1016/j.talanta.2019.06.050

PMID:31357375

Abstract

Viscosity is a key factor that determines the diffusion-controlled processes in biological systems. The matrix of mitochondria contains various enzymes and other proteins with high density, and the diffusion of which are severely restricted by the cristae, making it the most crowded place in the cells. Herein, we reported a new near-infrared probe NV-1 with increased Stokes shift for monitoring viscosity changes in mitochondria. A remarkable increase of the fluorescence was observed in glycerol compared with which was observed in methanol at 744 nm. The probe was applied for measuring viscosity changes not only in mitochondria, but also in vivo (in zebra fishes and mice).

摘要

黏度是决定生物系统扩散控制过程的关键因素。线粒体基质中含有各种高密度的酶和其他蛋白质，其扩散受到嵴的严重限制，使其成为细胞中最拥挤的地方。在这里，我们报道了一种新型近红外探针 NV-1，其斯托克斯位移增加，可用于监测线粒体中的黏度变化。与在甲醇中观察到的相比，在甘油中观察到了在 744nm 处的荧光显著增加。该探针不仅可用于测量线粒体中的黏度变化，还可用于活体（斑马鱼和小鼠）。