Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
J Mater Chem B. 2020 Oct 7;8(38):8838-8844. doi: 10.1039/d0tb01329c.
The viscosity of lysosomes plays a significant role in modulating biological processes and reflects the status and function of this kind of organelle, e.g., locations, morphologies, and components. Herein, we constructed a novel near-infrared (NIR) lysosome-targeting viscosity probe, Lyso-cy, for monitoring viscosity changes in biological systems. The Lyso-cy probe showed very strong fluorescence emission at around 710 nm in viscous media. The fluorescence intensity of Lyso-cy increased 122-fold from when in water to when in 95% glycerol. Moreover, Lyso-cy proved to be an ideal lysosome-targeting tracer for monitoring fluctuations in the viscosity of a living cell with high spatial and temporal resolution under laser confocal microscopy.
溶酶体的黏度在调节生物过程中起着重要作用，反映了这种细胞器的状态和功能，例如位置、形态和组成。在此，我们构建了一种新型的近红外（NIR）溶酶体靶向黏度探针 Lyso-cy，用于监测生物系统中的黏度变化。在粘性介质中，Lyso-cy 探针在约 710nm 处显示出非常强的荧光发射。当 Lyso-cy 从水中转移到 95%甘油中时，其荧光强度增加了 122 倍。此外，Lyso-cy 被证明是一种理想的溶酶体靶向示踪剂，可在激光共聚焦显微镜下以高时空分辨率监测活细胞中黏度的波动。
