Grześkowiak Łukasz, Dadi Temesgen Hailemariam, Zentek Jürgen, Vahjen Wilfried
Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise Strasse 49, 14195 Berlin, Germany.
Microorganisms. 2019 Jul 26;7(8):218. doi: 10.3390/microorganisms7080218.
(syn. ) is considered a pioneer colonizer and may cause gut infection in neonatal piglets. The aim of this study was to explore the microbiota- associations in pigs. We used the DNA from the faeces of four sows collected during the periparturient period and from two to three of their piglets (collected weekly until nine weeks of age) for the determination of bacterial community composition (sequencing) and concentration (qPCR). Furthermore, -negative faeces were enriched in a growth medium, followed by qPCR to verify the presence of this bacterium. -sensu-stricto-1 and spp. predominated the gut microbiota of the sows and their offspring. was detected at least once in the faeces of all sows during the entire sampling period, albeit at low concentrations. Suckling piglets harboured in high concentrations (up to log 9.29 copy number/g faeces), which gradually decreased as the piglets aged. Enrichment revealed the presence of in previously -negative sow and offspring faeces. In suckling piglets, the level was negatively correlated with carbohydrate-fermenting bacteria, and it was positively associated with potential pathogens. Shannon and richness diversity indices were negatively associated with the counts in suckling piglets. This study showed that gut microbiota seems to set conditions for colonisation resistance against in the offspring. However, this conclusion requires further research to include host-specific factors.
（同义词）被认为是先锋定植菌，可能导致新生仔猪肠道感染。本研究的目的是探索猪体内的微生物群关联。我们使用了围产期收集的4头母猪及其2至3头仔猪（每周收集直至9周龄）粪便中的DNA，用于测定细菌群落组成（测序）和[细菌名称]浓度（定量聚合酶链反应）。此外，将[细菌名称]阴性粪便在生长培养基中富集，随后进行定量聚合酶链反应以验证该细菌的存在。[细菌名称]严格意义种1和[细菌名称]属在母猪及其后代的肠道微生物群中占主导地位。在整个采样期间，所有母猪的粪便中至少检测到一次[细菌名称]，尽管浓度较低。哺乳仔猪体内[细菌名称]浓度较高（高达每克粪便9.29对数拷贝数），随着仔猪年龄增长逐渐下降。富集显示在先前[细菌名称]阴性的母猪和后代粪便中存在[细菌名称]。在哺乳仔猪中，[细菌名称]水平与碳水化合物发酵细菌呈负相关，与潜在病原体呈正相关。香农多样性指数和丰富度多样性指数与哺乳仔猪中的[细菌名称]数量呈负相关。本研究表明，肠道微生物群似乎为后代抵抗[细菌名称]定植设定了条件。然而，这一结论需要进一步研究以纳入宿主特异性因素。
