Savic Gajic Ivana, Savic Ivan, Boskov Ivana, Žerajić Stanko, Markovic Ivana, Gajic Dragoljub
Faculty of Technology, University of Nis, Bulevar oslobodjenja 124, 16000 Leskovac, Serbia.
Technical Faculty, University of Belgrade, Vojske Jugoslavije 12, 19210 Bor, Serbia.
Antioxidants (Basel). 2019 Jul 27;8(8):248. doi: 10.3390/antiox8080248.
The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust () flowers using central composite design. The ethanol concentration (33-67%), extraction temperature (33-67 °C), and extraction time (17-33 min) were analyzed as the factors that impact the total phenolic content. The liquid-to-solid ratio of 10 cm g was the same during extractions. The optimal conditions were found to be 59 °C, 60% (v/v) ethanol, and extraction time of 30 min. The total phenolic content (TPC = 3.12 g 100 g dry plant material) and antioxidant activity (IC = 120.5 µg cm) of the extract obtained by ultrasound-assisted extraction were compared with those obtained by maceration (TPC = 2.54 g 100 g dry plant material, IC = 150.6 µg cm) and Soxhlet extraction (TPC = 3.22 g 100 g dry plant material, IC = 204.2 µg cm). The ultrasound-assisted extraction gave higher total phenolic content and better antioxidant activity for shorter extraction time so that it represents the technique of choice for the extraction of phenolic compounds. The obtained extract, as the source of antioxidants, can be applied in the pharmaceutical and food industries.
本研究的目的是采用中心复合设计优化刺槐花花中酚类化合物的超声辅助提取工艺。分析了乙醇浓度（33% - 67%）、提取温度（33 - 67℃）和提取时间（17 - 33分钟）对总酚含量的影响因素。提取过程中固液比为10 cm/g保持不变。发现最佳条件为59℃、60%（v/v）乙醇和30分钟的提取时间。将超声辅助提取得到的提取物的总酚含量（TPC = 3.12 g/100 g干植物材料）和抗氧化活性（IC = 120.5 μg/cm）与浸渍法（TPC = 2.54 g/100 g干植物材料，IC = 150.6 μg/cm）和索氏提取法（TPC = 3.22 g/100 g干植物材料，IC = 204.2 μg/cm）得到的结果进行了比较。超声辅助提取在较短的提取时间内具有更高的总酚含量和更好的抗氧化活性，因此它是提取酚类化合物的首选技术。所获得的提取物作为抗氧化剂来源，可应用于制药和食品工业。
