Kiani Ahmadreza, Torabi Payam, Mousavi Zeinab E
Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111 Iran.
Bioprocessing and Biodetection Laboratory, Department of Food Science and Technology, University of Tehran, Karaj, 31587-77871 Iran.
J Food Sci Technol. 2024 Oct;61(10):1930-1942. doi: 10.1007/s13197-024-05969-3. Epub 2024 Mar 18.
This research aims to maximize the extraction of phenolic compounds (PCs) from almond hull waste (AHW) using an ultrasound-assisted extraction (UAE) method, detect the PCs, and investigate the antimicrobial activity of the extracts against pathogens and interactions with a probiotic bacterium. The impact of various parameters including sonication amplitude, solvent/AHW ratio (mL/g), and extraction time on the total phenolic content (TPC) was investigated and the optimized extraction conditions were determined. The AHW extracts' minimum bacterial concentration (MBC) and minimum inhibitory concentration (MIC) against , , and were assessed. In optimal UAE conditions total PCs of 47.37 ± 0.24 mg gallic acid equivalent (GAE)/g dry weight (DW) were extracted. The HPLC analysis revealed that the flavonoid rutin, as well as -coumaric acid and rosmarinic acid, were only seen in the extracts obtained by UAE. Furthermore, -coumaric acid emerged as the most prevalent PC in the UAE extract. Antimicrobial activity analysis showed that UAE extracts exhibited higher effects in inhibiting the growth of , , and . The use of UAE treatment resulted in the extraction of a diverse range of PCs with increased antioxidant capacity and antimicrobial activity.
The online version contains supplementary material available at 10.1007/s13197-024-05969-3.
本研究旨在利用超声辅助提取（UAE）方法最大限度地从杏仁壳废料（AHW）中提取酚类化合物（PCs），检测这些PCs，并研究提取物对病原体的抗菌活性以及与益生菌的相互作用。研究了超声振幅、溶剂/AHW比例（mL/g）和提取时间等各种参数对总酚含量（TPC）的影响，并确定了优化的提取条件。评估了AHW提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌的最低杀菌浓度（MBC）和最低抑菌浓度（MIC）。在最佳UAE条件下，提取的总PCs为47.37±0.24毫克没食子酸当量（GAE）/克干重（DW）。高效液相色谱分析表明，黄酮类芦丁以及对香豆酸和迷迭香酸仅在通过UAE获得的提取物中出现。此外，对香豆酸是UAE提取物中最普遍的PC。抗菌活性分析表明，UAE提取物在抑制金黄色葡萄球菌、大肠杆菌和白色念珠菌的生长方面表现出更高的效果。使用UAE处理导致提取出多种具有增强抗氧化能力和抗菌活性的PC。
在线版本包含可在10.1007/s13197-024-05969-3获取的补充材料。
