Chi Cheng, Giri Sib Sankar, Jun Jin Woo, Kim Hyoun Joong, Kim Sang Wha, Yun Saekil, Park Se Chang
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea.
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea.
Fish Shellfish Immunol. 2017 Jun;65:111-117. doi: 10.1016/j.fsi.2017.03.031. Epub 2017 Mar 18.
Okadaic acid (OA) is produced by dinoflagellates during harmful algal blooms and is a diarrhetic shellfish-poisoning (DSP) toxin. This toxin is particularly problematic for bivalves that are cultured for human consumption. This study aimed to reveal the effects of exposure to OA on the non-specific immune responses of bay scallop, Argopecten irradians. Various immunological parameters (superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (ALP), lysozyme activities, and total protein level) were assessed in the hemolymph of bay scallops at 3, 6, 12, 24, and 48 h post-exposure (hpe) to different concentrations (50, 100, and 500 nM) of OA. Moreover, the expression of immune system-related genes (MnSOD, PrxV, PGRP, and BD) was also measured. Results showed that SOD and ACP activities were decreased between 12 and 48 hpe. The ALP, lysozyme activities, and total protein levels were also modulated after exposure to different concentrations of OA. The expression of immune-system-related genes was also assessed at different time points during the exposure period. Overall, our results suggest that the exposure to OA had negative effects on the antioxidant and non-specific immune responses, and even disrupted the metabolism of bay scallops, making them more vulnerable to environmental stress-inducing agents; they provide a better understanding of the response status of bivalves against DSP toxins.
冈田酸（OA）由有害藻华期间的双鞭毛藻产生，是一种腹泻性贝类毒素（DSP）。这种毒素对养殖供人类食用的双壳贝类尤其成问题。本研究旨在揭示暴露于OA对海湾扇贝（Argopecten irradians）非特异性免疫反应的影响。在暴露于不同浓度（50、100和500 nM）OA后的3、6、12、24和48小时（hpe），评估了海湾扇贝血淋巴中的各种免疫参数（超氧化物歧化酶（SOD）、酸性磷酸酶（ACP）、碱性磷酸酶（ALP）、溶菌酶活性和总蛋白水平）。此外，还测量了免疫系统相关基因（MnSOD、PrxV、PGRP和BD）的表达。结果表明，在暴露后12至48小时期间，SOD和ACP活性降低。暴露于不同浓度的OA后，ALP、溶菌酶活性和总蛋白水平也受到调节。在暴露期间的不同时间点也评估了免疫系统相关基因的表达。总体而言，我们的结果表明，暴露于OA对抗氧化和非特异性免疫反应有负面影响，甚至扰乱了海湾扇贝的代谢，使其更容易受到环境应激诱导剂的影响；它们有助于更好地了解双壳贝类对DSP毒素的反应状态。
