Chao Wen-Wan, Chen Shu-Ju, Peng Hui-Chen, Liao Jiunn-Wang, Chou Su-Tze
Department of Nutrition and Health Sciences, Kainan University, Taoyuan 33857, Taiwan.
Department of Food and Nutrition, Providence University, Taichung 43301, Taiwan.
Antioxidants (Basel). 2019 Jul 28;8(8):251. doi: 10.3390/antiox8080251.
(1) Background: E. Walther is a traditional Chinese herbal medicine. In our previous study, 50% ethanolic extracts (GE50) demonstrated good antioxidant activity. (2) Methods: To investigate the hepatoprotective effects of GE50 on ethanol and carbon tetrachloride (CCl) co-induced hepatic damage in rats, Sprague-Dawley rats were randomly divided into five groups (Control group; GE50 group, 0.25 g/100 g BW; EC group: Ethanol + CCl, 1.25 mL 50% ethanol and 0.1 mL 20% CCl/100 g BW; EC + GE50 group: Ethanol + CCl + GE50; EC + silymarin group: ethanol + CCl + silymarin, 20 mg/100 g BW) for six consecutive weeks. (3) Results: Compared with the control group, EC group significantly elevated the serum aspartate aminotransferase (AST), alanine aminitransferase (ALT), and lactate dehydrogenase (LDH). However, GE50 or silymarin treatment effectively reversed these changes. GE50 had a significant protective effect against ethanol + CCl induced lipid peroxidation and increased the levels of glutathione (GSH), vitamin C, E, total antioxidant status (TAS), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferases (GST). Furthermore, in EC focal group, slight fat droplet infiltration was observed in the livers, while in the GE50 or silymarin treatment groups, decreased fat droplet infiltration. HPLC phytochemical profile of GE50 revealed the presence of gallic acid, flavone, genistin, daidzin, and quercetin. (4) Conclusions: The hepatoprotective activity of GE50 is proposed to occur through the synergic effects of its chemical component, namely, gallic acid, flavone, genistin, daidzin, and quercetin. Hence, can be used as a complementary and alternative therapy in the prevention of alcohol + CCl-induced liver injury.
(1) 背景：E. Walther是一种传统中药。在我们之前的研究中，50%乙醇提取物（GE50）表现出良好的抗氧化活性。(2) 方法：为研究GE50对乙醇和四氯化碳（CCl）联合诱导的大鼠肝损伤的保肝作用，将Sprague-Dawley大鼠随机分为五组（对照组；GE50组，0.25 g/100 g体重；EC组：乙醇+CCl，1.25 mL 50%乙醇和0.1 mL 20% CCl/100 g体重；EC+GE50组：乙醇+CCl+GE50；EC+水飞蓟宾组：乙醇+CCl+水飞蓟宾，20 mg/100 g体重），连续六周。(3) 结果：与对照组相比，EC组血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和乳酸脱氢酶（LDH）显著升高。然而，GE50或水飞蓟宾治疗有效逆转了这些变化。GE50对乙醇+CCl诱导的脂质过氧化具有显著保护作用，并提高了谷胱甘肽（GSH）、维生素C、E、总抗氧化状态（TAS）水平以及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）的活性。此外，在EC病灶组中，肝脏观察到轻微的脂肪滴浸润，而在GE50或水飞蓟宾治疗组中，脂肪滴浸润减少。GE50的HPLC植物化学图谱显示存在没食子酸、黄酮、染料木苷、大豆苷和槲皮素。(4) 结论：GE50的保肝活性被认为是通过其化学成分没食子酸、黄酮、染料木苷、大豆苷和槲皮素的协同作用产生的。因此，可作为预防酒精+CCl诱导的肝损伤的补充和替代疗法。
