Division of Life Science & State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology (HKUST), Clearwater Bay, Hong Kong, PRC.
Cold Spring Harb Perspect Biol. 2020 Jan 2;12(1):a035170. doi: 10.1101/cshperspect.a035170.
Since the identification of nicotinic acid adenine dinucleotide phosphate (NAADP) and its putative target, the two-pore channel (TPC), the NAADP/TPC/Ca signaling pathway has been reported to play a role in a diverse range of functions in a variety of different cell types. TPCs have also been associated with a number of diseases, which arise when their activity is perturbed. In addition, TPCs have been shown to play key roles in various embryological processes and during the differentiation of a variety of cell types. Here, we review the role of NAADP/TPC/Ca signaling during early embryonic development and cellular differentiation. We pay particular attention to the role of TPC2 in the development and maturation of early neuromuscular activity in zebrafish, and during the differentiation of isolated osteoclasts, endothelial cells, and keratinocytes. Our aim is to emphasize the conserved features of TPC-mediated Ca signaling in a number of selected examples.
自从鉴定出烟酰胺腺嘌呤二核苷酸磷酸（NAADP）及其假定靶点——双孔通道（TPC）以来，NAADP/TPC/Ca 信号通路已被报道在多种不同细胞类型的多种功能中发挥作用。TPC 也与许多疾病有关，当它们的活性受到干扰时，这些疾病就会出现。此外，TPC 已被证明在各种胚胎发育过程和多种细胞类型的分化中发挥关键作用。在这里，我们回顾了 NAADP/TPC/Ca 信号在早期胚胎发育和细胞分化中的作用。我们特别关注 TPC2 在斑马鱼早期神经肌肉活动的发育和成熟中的作用，以及在分离的破骨细胞、内皮细胞和角质细胞的分化中的作用。我们的目的是强调 TPC 介导的 Ca 信号在一些选定的例子中的保守特征。
