College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
Food Funct. 2019 Aug 1;10(8):5046-5058. doi: 10.1039/c9fo00686a. Epub 2019 Jul 30.
Human and bovine milk fat globule membrane (MFGM) proteins have been identified and characterized; however, their glycosylation during lactation remains unclear. We adopted a glycoproteomics approach to profile and compare MFGM N-glycoproteomes in human and bovine milk during lactation. A total of 843, 718, 614, and 273 N-glycosite peptides corresponding to 465, 423, 334, and 176 glycoproteins were identified in human colostrum, human mature milk, bovine colostrum, and bovine mature milk, respectively. The biological functions of these MFGM N-glycoproteins were revealed through bioinformatics. Substantial differences were observed between human and bovine milk, and immune-related MFGM N-glycoproteins varied between colostrum and mature milk from both species. Our results expand current knowledge of MFGM N-glycoproteomes, and further demonstrate the complexity and biological functions of MFGM N-glycosylation. These data can provide references for the application of bovine MFGM N-glycoproteins in infant formula to resemble human milk and in functional foods.
人乳和牛乳脂肪球膜 (MFGM) 蛋白已被鉴定和表征；然而，其在哺乳期的糖基化仍不清楚。我们采用糖蛋白质组学方法来分析和比较哺乳期人乳和牛乳 MFGM N-糖蛋白组。在人初乳、人成熟乳、牛初乳和牛成熟乳中分别鉴定到 843、718、614 和 273 个分别对应 465、423、334 和 176 个糖蛋白的 N-糖基化肽。通过生物信息学揭示了这些 MFGM N-糖蛋白的生物学功能。人乳和牛乳之间存在显著差异，且两种物种的初乳和成熟乳中的免疫相关 MFGM N-糖蛋白也存在差异。我们的研究结果扩展了 MFGM N-糖蛋白组的现有知识，并进一步证明了 MFGM N-糖基化的复杂性和生物学功能。这些数据可为牛 MFGM N-糖蛋白在婴儿配方奶粉中的应用提供参考，以模仿人乳，并应用于功能性食品。
